Presynaptic Ca2+-activated K+ channels in glutamatergic hippocampal terminals and their role in spike repolarization and regulation of transmitter release

被引:284
作者
Hu, H
Shao, LR
Chavoshy, S
Gu, N
Trieb, M
Behrens, R
Laake, P
Pongs, O
Knaus, HG
Ottersen, OP
Storm, JF
机构
[1] Univ Oslo, Inst Physiol, N-0317 Oslo, Norway
[2] Univ Oslo, Inst Anat, N-0317 Oslo, Norway
[3] Univ Oslo, Inst Med Stat, N-0317 Oslo, Norway
[4] Inst Biochem Pharmacol, A-6020 Innsbruck, Austria
[5] Univ Hamburg, Zentrum Mol Neurobiol, Inst Neurale Signalverarbeitung, D-20246 Hamburg, Germany
关键词
calcium-activated potassium channels; BK channels; Slo; Maxi-K; presynaptic mechanisms; hippocampus; CA1; CA3; action potential repolarization; glutamatergic synapses; immunogold cytochemistry; BK-beta; 4; KCNMB4;
D O I
10.1523/JNEUROSCI.21-24-09585.2001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Large-conductance Ca2+-activated K+ channels (BK, also called Maxi-K or Slo channels) are widespread in the vertebrate nervous system, but their functional roles in synaptic transmission in the mammalian brain are largely unknown. By combining electrophysiology and immunogold cytochemistry, we demonstrate the existence of functional BK channels in presynaptic terminals in the hippocampus and compare their functional roles in somata and terminals of CA3 pyramidal cells. Double-labeling immunogold analysis with BK channel and glutamate receptor antibodies indicated that BK channels are targeted to the presynaptic membrane facing the synaptic cleft in terminals of Schaffer collaterals in stratum radiatum. Whole-cell, intracellular, and field-potential recordings from CA1 pyramidal cells showed that the presynaptic BK channels are activated by calcium influx and can contribute to repolarization of the presynaptic action potential (AP) and negative feedback control of Ca2+ influx and transmitter release. This was observed in the presence of 4-aminopyridine (4-AP, 40-100 muM), which broadened the presynaptic compound action potential. In contrast, the presynaptic BK channels did not contribute significantly to regulation of action potentials or transmitter release under basal experimental conditions, i.e., without 4-AP, even at high stimulation frequencies. This is unlike the situation in the parent cell bodies (CA3 pyramidal cells), where BK channels contribute strongly to action potential repolarization. These results indicate that the functional role of BK channels depends on their subcellular localization.
引用
收藏
页码:9585 / 9597
页数:13
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