Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p

被引：126

作者：

Zenke, FT ^{[1
]}

Engels, R ^{[1
]}

Vollenbroich, V ^{[1
]}

Meyer, J ^{[1
]}

Hollenberg, CP ^{[1
]}

Breunig, KD ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST MIKROBIOL,D-40225 DUSSELDORF,GERMANY

来源：

SCIENCE | 1996年 / 272卷 / 5268期

关键词：

D O I：

10.1126/science.272.5268.1662

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Yeast galactokinase (Gal1p) is an enzyme and a regulator of transcription. In addition to phosphorylating galactose, Gal1p activates Gal4p, the activator of GAL genes, but the mechanism of this regulation has been unclear. Here, biochemical and genetic evidence is presented to show that Gal1p activates Gal4p by direct interaction with the Gal4p inhibitor Gal80p. interaction requires galactose, adenosine triphosphate, and the regulatory function of Gal1p. These data indicate that Gal1p-Gal80p complex formation results in the inactivation of Gal80p, thereby transmitting the galactose signal to Gal4p.

引用

页码：1662 / 1665

页数：4

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