Acetyl coenzyme A concentrations in plant tissues

被引：25

作者：

Tumaney, AW ^{[1
]}

Ohlrogge, JB ^{[1
]}

Pollard, M ^{[1
]}

机构：

[1] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2004年 / 161卷 / 04期

关键词：

acetyl coenzyme A; Arabidopsis thaiiana; castor (Ricinus communis); Euonymus alata; leaves; seeds; spinach (Spinacia oleracea); rapeseed (Brassica napus);

D O I：

10.1078/0176-1617-01258

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Despite the importance of acetyl coenzyme A in many facets of metabolism and the availability of methods for estimation of its concentration, data for acetyl-CoA concentrations in plant tissues have been very scarce. A method using reversed phase HPLC for the quantitative estimation of acetylCoA was applied to a variety of plant tissues. In three different developing oilseeds the bulk acetylCoA concentration ranged from 5 to 25 nmoles/g fresh weight. In Arabidopsis thaliana leaves it was 5 nmoles/g fresh weight, and in Spinacia oleracea leaves 6.8 nmoles/g fresh weight. Immediate quenching of the harvested tissue in liquid nitrogen is needed to obtain high recoveries of acetylCoA.

引用

页码：485 / 488

页数：4