Self renewal of embryonic stem cells in the absence of feeder cells and exogenous leukaemia inhibitory factor

被引:15
作者
Berger, CN [1 ]
Sturm, KS [1 ]
机构
[1] CHILDRENS MED RES INST,WENTWORTHVILLE,NSW 2145,AUSTRALIA
关键词
anti-LIF antibodies; blastocyst injection chimaeras; ES cells; beta-galactosidase transgenics; leukaemia inhibitory factor; pluripotency; self renewal;
D O I
10.3109/08977199709021517
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To evaluate the role of leukaemia inhibitory factor (LIF) for maintaining pluripotent embryonic stem (ES) cells in culture, we established several exogenous LIF-independent ES cell Lines by continuous passaging in culture, The newly established ES cells, Kli and CBli, sustained their growth and remained undifferentiated in LIF-deficient medium, Analysis of chimaeric animals, produced with the beta-galactosidase transgenic Kli ES cells, revealed that LIF-independent ES cells can contribute to all embryonic germ layers, There was no detectable LIF protein in ES cell conditioned medium, and no upregulation of LIF mRNA was found, The addition of neutralising anti-LIF antibodies was not sufficient to abrogate the self renewal of the Kli ES cells, These studies suggest that the signalling pathway involving diffusible LIF can be bypassed for maintaining the pluripotency in culture, and indicate a considerable heterogeneity in growth factor dependence and differentiation of different ES cells.
引用
收藏
页码:145 / &
页数:17
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