Sampling considerations for herd-level measurement of faecal Escherichia coli antimicrobial resistance in finisher pigs

被引：30

作者：

Dunlop, RH

McEwen, SA ^{[1
]}

Meek, AH

Friendship, RM

Black, WD

Clarke, RC

机构：

[1] Univ Guelph, Dept Populat Med, Guelph, ON N1G 2W1, Canada

[2] Univ Guelph, Dept Biomed Sci, Guelph, ON N1G 2W1, Canada

[3] Hlth Canada, Hlth Anim Lab, Guelph, ON, Canada

来源：

EPIDEMIOLOGY AND INFECTION | 1999年 / 122卷 / 03期

关键词：

D O I：

10.1017/S0950268899002411

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The objective of this study was to determine the most efficient means of sampling faeces of finisher pigs for accurate and precise farm-level estimates of antimicrobial resistance among faecal Escherichia coli. Resistance to tetracycline and gentamicin of 8250 isolates of E. coli from 55 finisher pigs on one farm was measured with a hydrophobic grid membrane filter method. The between-pig, within-pen component of variance in resistance was large (97.5 %), while between-pen, within-room and between-room components were small (2.5 % and 0 %, respectively). Using these resistance data, the abilities of two sampling strategies to estimate prevalence were modelled with a Monte Carlo 'bootstrap' procedure. Compositing faecal samples from several pigs before testing produced unbiased and precise estimates of prevalence and is simpler technically than individual animal testing.

引用

页码：485 / 496

页数：12

共 30 条

[11] STATISTICAL CONSIDERATIONS IN SAMPLING OF ESCHERICHIA-COLI FROM INTESTINAL SOURCES FOR SEROTYPING [J].

HEDGES, AJ ;

HOWE, K ;

LINTON, AH .

JOURNAL OF APPLIED BACTERIOLOGY, 1977, 43 (02) :271-280

[12] OLAQUINDOX RESISTANCE IN THE COLIFORM FLORA OF PIGS AND THEIR ENVIRONMENT - AN ECOLOGICAL STUDY [J].

HEDGES, AJ ;

LINTON, AH .

JOURNAL OF APPLIED BACTERIOLOGY, 1988, 64 (05) :429-443

[13]

Helrich K, 1990, OFFICIAL METHODS ANA, VI

[14] THE ECOLOGY OF ESCHERICHIA-COLI IN ANIMALS INCLUDING MAN WITH PARTICULAR REFERENCE TO DRUG-RESISTANCE [J].

HINTON, M .

VETERINARY RECORD, 1986, 119 (17) :420-426

[15] THE EFFECTS OF OXYTETRACYCLINE ON THE INTESTINAL ESCHERICHIA-COLI FLORA OF NEWLY WEANED PIGS [J].

HINTON, M ;

HAMPSON, DJ ;

HAMPSON, E ;

LINTON, AH .

JOURNAL OF HYGIENE, 1985, 95 (01) :77-85

[16] SPREAD OF PLASMID-MEDIATED NOURSEOTHRICIN RESISTANCE DUE TO ANTIBIOTIC USE IN ANIMAL HUSBANDRY [J].

HUMMEL, R ;

TSCHAPE, H ;

WITTE, W .

JOURNAL OF BASIC MICROBIOLOGY, 1986, 26 (08) :461-466

[17]

JEWERS K, 1989, J APPL BACTERIOL S, P105

[18] MICROBIAL COMMUNITY ANALYSIS IN INCOMPLETELY OR DESTRUCTIVELY SAMPLED SYSTEMS [J].

KINKEL, LL ;

NORDHEIM, EV ;

ANDREWS, JH .

MICROBIAL ECOLOGY, 1992, 24 (03) :227-242

[19] ANTIBIOTIC-RESISTANCE OF FECAL COLIFORMS AFTER LONG-TERM WITHDRAWAL OF THERAPEUTIC AND SUBTHERAPEUTIC ANTIBIOTIC USE IN A SWINE HERD [J].

LANGLOIS, BE ;

CROMWELL, GL ;

STAHLY, TS ;

DAWSON, KA ;

HAYS, VW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (06) :1433-1434

[20] ANTIBIOTIC-RESISTANCE OF FECAL COLIFORMS FROM SWINE FED SUBTHERAPEUTIC AND THERAPEUTIC LEVELS OF CHLORTETRACYCLINE [J].

LANGLOIS, BE ;

DAWSON, KA ;

STAHLY, TS ;

CROMWELL, GL .

JOURNAL OF ANIMAL SCIENCE, 1984, 58 (03) :666-674

← 1 2 3 →