Memory effect of mobile phase additives in chiral separations on a Chiralpak AD column

被引：52

作者：

Ye, YK ^{[1
]}

Lord, B ^{[1
]}

Stringham, RW ^{[1
]}

机构：

[1] Dupont Merck Pharmaceut Co, Chem Proc R&D, Chambers Works, Deepwater, NJ 08023 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 945卷 / 1-2期

关键词：

memory effect; mobile phase composition; enantiomer separation; amino acids;

D O I：

10.1016/S0021-9673(01)01491-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using chiral probes shown to be sensitive to the presence of mobile phase additives, a memory effect for these additives by an amylosic column was demonstrated. Exposure to these additives gave prolonged chromatographic performance changes even after their removal from the mobile phase. This finding is consistent with strong binding of the additives to the stationary phase. A procedure to remove bound additives was developed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：139 / 146

页数：8

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