Inhibition of vascular endothelial growth factor expression by TRUE gene silencing

被引：21

作者：

Elbarbary, Reyad A. ^{[1
]}

Takaku, Hiroaki ^{[1
]}

Tamura, Masato ^{[2
]}

Nashimoto, Masayuki ^{[1
]}

机构：

[1] Niigata Univ Pharm & Appl Life Sci, Dept Appl Life Sci, Niigata 9568603, Japan

[2] Hokkaido Univ, Dept Biochem & Mol Biol, Grad Sch Dent Med, Sapporo, Hokkaido 0608586, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 04期

关键词：

5 '-Half-tRNA; sgRNA; tRNase Z(L); TRUE gene silencing; VEGF; 3' PROCESSING ENDORIBONUCLEASE; TRANSFER-RNA; TARGETING VEGF; OCULAR NEOVASCULARIZATION; TRNASE-ZL; CLEAVAGE; SIRNA; ANGIOGENESIS; SUPPRESSION; HEPTAMERS;

D O I：

10.1016/j.bbrc.2008.12.173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pathogenic angiogenesis in various diseases including cancer, autoimmune diseases, and age-related macular degeneration is thought to be regressed with anti-angiogenic drugs. TRUE gene silencing is a new technology to eliminate a specific mRNA using synthetic sgRNA and cellular tRNase Z(L). To discover anti-angiogenic sgRNAs, we applied TRUE silencing to the VEGF gene. We examined eight sgRNAs for efficacy in targeting exogenous human VEGF mRNA. Many of them worked efficiently in 293 and HeLa cells. Two of them downregulated the endogeneous VEGF gene expression in HeLa cells very efficiently, and the efficacy of these two sgRNAs surpassed that of siRNA extremely. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：924 / 927

页数：4

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