Survey of nutrients and quality assessment of crab paste by 1H NMR spectroscopy and multivariate data analysis

被引：19

作者：

Ye YangFang ^{[1
]}

Zhang LiMin ^{[2
]}

Tang HuiRu ^{[2
]}

Yan XiaoJun ^{[1
]}

机构：

[1] Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China

[2] Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2012年 / 57卷 / 25期

基金：

中国国家自然科学基金;

关键词：

crab paste; Portunus trituberculatus; composition; nuclear magnetic resonance (NMR); multivariate data analysis (MDA); quality assessment; COMPOSITIONAL CHARACTERISTICS; DISCRIMINATION; SPECTROMETRY; METABONOMICS; EXTRACTS; FISH;

D O I：

10.1007/s11434-012-5119-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Crab paste is a traditional and popular food for people in the coastal area of China. Visual inspection is currently the only method of quality assessment. We developed a new analytical method of a comprehensive survey of nutrients and quality assessment for crab paste based on a combination of H-1 NMR spectroscopy with multivariate data analysis. The aqueous extract of crab paste was dominated by amino acids, sugars, carboxylic acids, nucleotides and amines (including 19 first-time-reported compounds such as choline, uracil and guanosine). Two grades of crab paste had significant compositional differences in terms of amino acids, lactate, N-acetylglutamate, choline, dimethylamine, uridine, 1-methylnicotinamide and 2-pyridienmethanol. These results provided important information on the grade-dependence of crab-paste composition, and demonstrated that NMR-MDA was effective not only for the comprehensive survey of nutrients, but also for quality assessment of crab paste.

引用

页码：3353 / 3362

页数：10

共 36 条

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