Isolation and characterization of a xylose-dependent promoter from Caulobacter crescentus

被引:118
作者
Meisenzahl, AC [1 ]
Shapiro, L [1 ]
Jenal, U [1 ]
机构
[1] STANFORD UNIV, SCH MED, DEPT DEV BIOL, STANFORD, CA 94305 USA
关键词
D O I
10.1128/jb.179.3.592-600.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An inducible promoter is a useful tool for the controlled expression of a given gene, Accordingly, we identified, cloned, and sequenced a chromosomal locus, xylX, from Caulobacter crescentus which is required for growth on xylose as the sole carbon source and showed that transcription from a single site is dependent on the presence of xylose in the growth medium, P-xylX promoter activity was determined as a function of the composition of the growth medium both in single copy and on a plasmid using different reporter genes. One hundred micromolar exogenously added xylose was required for maximal induction of P-xylX in a strain that is unable to metabolize xylose, P-xylX activity was induced immediately after the addition of xylose and repressed almost completely when xylose was removed from the growth medium, In addition to the strong transcriptional control, the expression of xylX is also regulated on the translational level.
引用
收藏
页码:592 / 600
页数:9
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