Proteomics as a tool for assessment of therapeutics in transfusion medicine:: evaluation of prothrombin complex concentrates

被引:25
作者
Brigulla, M
Thiele, T
Scharf, C
Breitner-Ruddock, S
Venz, S
Völker, U
Greinacher, A
机构
[1] Univ Greifswald, Inst Immunol & Transfus Med, D-17489 Greifswald, Germany
[2] Univ Greifswald, Lab Funct Genom, Greifswald, Germany
[3] Paul Ehrlich Inst, D-6070 Langen, Germany
关键词
D O I
10.1111/j.1537-2995.2006.00732.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND: Proteomic technologies are evolving tools to analyze complex protein patterns that to date have been rarely applied to transfusion medicine. The analysis of prothrombin complex concentrates (PCCs) was used as a model to evaluate to what extent these technologies can detect differences in blood-derived therapeutics beyond that of standard quality control. STUDY DESIGN AND METHODS: Three PCCs (two batches each) were individually analyzed for differences in protein content by functional assays, two-dimensional gel electrophoresis, and mass spectrometry. The results were compared to a pool of 72 normal plasma samples. RESULTS: A highly complex protein pattern was found that varied considerably among the three PCCs: 192 spots comprising 40 different proteins were identified. Factor (F) IX activities of the three PCCs were comparable, but their F IX protein contents differed considerably. Many proteins were present in multiple spots (e.g. FII, FX, protein C, vitronectin), indicating a high degree of posttranslational modifications. In comparison with untreated pooled plasma, PCCs displayed several low-molecular-weight variants of proteins that likely constitute potential degradation products. CONCLUSION: Proteomic technologies allow the identification of potentially modified proteins in clotting factor concentrates, indicating that they could become a useful tool for transfusion medicine to assess the impact of processing on the integrity of blood-derived therapeutics.
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页码:377 / 385
页数:9
相关论文
共 22 条
  • [21] Detection of HIV-1 and HCV infections among antibody-negative blood donors by nucleic acid-amplification testing
    Stramer, SL
    Glynn, SA
    Kleinman, SH
    Strong, DM
    Caglioti, S
    Wright, DJ
    Dodd, RY
    Busch, MP
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (08) : 760 - 768
  • [22] TEMPERLEY IJ, 1992, LANCET, V340, P1466