Determination of Phenolic Acids in Danshen Preparations by LC with Chemiluminescence Detection

被引：10

作者：

Chang, Yan-Xu ^{[1
]}

Ding, Xiao-Ping ^{[1
]}

Qi, Jin ^{[1
]}

Cao, Jun ^{[1
]}

Kang, Li-Yuan ^{[2
]}

Zhu, Dan-Ni ^{[1
]}

Zhang, Bo-Li ^{[1
,2
]}

Yu, Bo-Yang ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Complex Prescript TCM, Nanjing 210009, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, Key Lab Prescript, Natl Educ Minist China, Tianjin 300193, Peoples R China

来源：

CHROMATOGRAPHIA | 2009年 / 69卷 / 3-4期

关键词：

Column liquid chromatography; Chemiluminescence detection; Phenolic acids; Danshen preparations; PERFORMANCE LIQUID-CHROMATOGRAPHY; SALVIA-MILTIORRHIZA; MASS-SPECTROMETRY;

D O I：

10.1365/s10337-008-0876-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive method has been developed for the simultaneous determination of eight phenolic acids in Danshen preparations based on liquid chromatography with chemiluminescence detection. Chemiluminescence parameters including flow rate, buffer pH, the concentration of luminescent and reactive solutions, were optimized. The analytical performance of the optimized luminol-H2O2 detection was compared with those of luminol-pyrogallol and UV detection. Under the optimized conditions, the method was validated with respect to linearity, precision, limits of detection and quantification. The method offers an attractive alternative to be used to evaluate the quality of Danshen preparations.

引用

页码：319 / 323

页数：5

共 17 条

[1]

Cai M, 2001, J CHIN PHARM U, V32, P130

[2] Cardiovascular effects of Danshen [J].

Cheng, Tsung O. .

INTERNATIONAL JOURNAL OF CARDIOLOGY, 2007, 121 (01) :9-22

[3] Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection [J].

Costin, Jason W. ;

Lewis, Simon W. ;

Purcell, Stuart D. ;

Waddell, Lucy R. ;

Francis, Paul S. ;

Barrnett, Neil W. .

ANALYTICA CHIMICA ACTA, 2007, 597 (01) :19-23

[4] Comparison of cardioprotective effects using ramipril and DanShen for the treatment of acute myocardial infarction in rats [J].

Ji, XY ;

Tan, BKH ;

Zhu, YC ;

Linz, W ;

Zhu, YZ .

LIFE SCIENCES, 2003, 73 (11) :1413-1426

[5]

Li J., 1993, Acta Pharmaceutica Sinica, V28, P543

[6] Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry [J].

Li, XC ;

Yu, C ;

Cai, YB ;

Liu, GY ;

Jia, JY ;

Wang, YP .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 820 (01) :41-47

[7]

Li YS, 2006, CHIN J INTEGR MED CA, V4, P791

[8] Simultaneous quantification of six major phenolic acids in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations by HPLC-DAD method [J].

Liu, AH ;

Li, L ;

Xu, M ;

Lin, YH ;

Guo, HZ ;

Guo, DA .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 41 (01) :48-56

[9] Detection, characterization and identification of phenolic acids in Danshen using high-performance. liquid chromatography with diode array detection and electrospray ionization mass spectrometry [J].

Liu, Ai-Hua ;

Guo, Hui ;

Ye, Min ;

Lin, Yan-Hua ;

Sun, Hang-Hao ;

Xu, Man ;

Guo, De-An .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1161 (1-2) :170-182

[10] Determination of four water-soluble compounds in Salvia miltiorrhiza Bunge by high-performance liquid chromatography with a coulometric electrode array system [J].

Ma, LJ ;

Zhang, XZ ;

Guo, H ;

Gan, YR .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 833 (02) :260-263

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