Identification and characterization of N-acylhomoserine lactone-acylase from the fish intestinal Shewanella sp strain MIB015

被引：72

作者：

Morohoshi, Tomohiro ^{[1
]}

Nakazawa, Shigehisa ^{[1
]}

Ebata, Atsushi ^{[1
]}

Kato, Norlhlro ^{[1
]}

Ikeda, Tsukasa ^{[1
]}

机构：

[1] Utsunomiya Univ, Fac Engn, Dept Appl Chem, Utsunomiya, Tochigi 3218585, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 07期

关键词：

quorum sensing; acylhomoserine lactone; acylase; quorum quenching; Shewanella sp;

D O I：

10.1271/bbb.80139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many gram-negative bacteria. We have reported that Shewanella sp. strain MIB015 degrades AHLs. In the present study, we cloned the aac gene from MIB015 by PCR with specific primers based on the aac gene in Shewanella oneidensis strain MR-1, which showed high homology with the known AHL-acylases. Escherichia coli expressing Aac showed high degrading activity of AHLs with long acyl chains. HPLC analysis revealed that Aac worked as AHL-acylase, which hydrolyzed the amide bond of AHL. In addition, expression of Aac in fish pathogen Vibrio anguillarum markedly reduced AHL production and biofilm formation. In conclusion, this study indicates that Aac might be effective in quenching quorum sensing of fish pathogens.

引用

页码：1887 / 1893

页数：7

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