Primary microRNA transcripts are processed co-transcriptionally

被引：283

作者：

Morlando, Mariangela ^{[1
,2
,3
]}

Ballarino, Monica ^{[2
,3
]}

Gromak, Natalia ^{[1
]}

Pagano, Francesca ^{[2
,3
]}

Bozzoni, Irene ^{[2
,3
]}

Proudfoot, Nick J. ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Univ Roma La Sapienza, Dept Genet & Mol Biol, Inst Pasteur Cenci Bolognetti, I-00185 Rome, Italy

[3] Univ Roma La Sapienza, IBPM, I-00185 Rome, Italy

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 09期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

D O I：

10.1038/nsmb.1475

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

microRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II) -derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri- miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on both independently transcribed and intron-encoded miRNAs. We also show that both 5'-3' and 3'-5' exonucleases associate with the sites where co-transcriptional Drosha cleavage occurs, promoting intron degradation before splicing. We finally demonstrate that miRNAs can also derive from 3' flanking transcripts of Pol II genes. Our results demonstrate that multiple miRNA-containing transcripts are co-transcriptionally cleaved during their synthesis and suggest that exonucleolytic degradation from Drosha cleavage sites in pre-mRNAs may influence the splicing and maturation of numerous mRNAs.

引用

页码：902 / 909

页数：8

共 56 条

[51] Human 5′→3′ exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites [J].