Determination of creatine and guanidinoacetate by GC-MS Study of their stability in urine at different temperatures

被引：9

作者：

Angel Prieto, Jose ^{[1
]}

Andrade, Fernando ^{[1
]}

Martin, Sergio ^{[1
]}

Sanjurjo, Pablo ^{[1
]}

Elorz, Javier ^{[2
]}

Aldamiz-Echevarria, Luis ^{[1
]}

机构：

[1] Cruces Hosp, Div Metab, Baracaldo 48903, Spain

[2] Basurto Hosp, Bilbao 48013, Spain

来源：

CLINICAL BIOCHEMISTRY | 2009年 / 42卷 / 1-2期

关键词：

Gas chromatography-mass spectrometry; Creatine; Guanidinoacetate; Urine; Stability; TANDEM MASS-SPECTROMETRY; DEFICIENCY; DIAGNOSIS; PLASMA; ACID;

D O I：

10.1016/j.clinbiochem.2008.10.001

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) as the silylating agent for GC-MS. Study of the stability of creatine and guanidinoacetate in urine. Design and methods: 22 urines were kept at RT, 4 degrees C and -30 degrees C for 15 days. Results: MTBSTFA produces a single chromatographic peak in contrast with other derivatizing agents. Creatine concentration increases at room temperature (326% on average), and at 4 degrees C (75%). However, detection decreases after freezing (-37%). Guanidinoacetate is stable, but decreases after freezing (-37%). Sonication before analysis is crucial to obtain repetitive results. Conclusions: A modified GC-MS method has been validated and the conditions for preservation of the urine have been established. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

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页码：125 / 128

页数：4

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