Altered gene expression in acute systemic inflammation detected by complete coverage of the human liver transcriptome

被引:24
作者
Coulouarn, C
Lefebvre, G
Derambure, C
Lequerre, T
Scotte, M
Francois, A
Cellier, D
Daveau, M
Salier, JP
机构
[1] Fac Med Pharm, INSERM, U519, F-76183 Rouen, France
[2] Fac Med Pharm, Inst Federatif Rech Multidisciplinaires Peptides, F-76183 Rouen, France
[3] CHU Rouen, Dept Pathol, Rouen, France
[4] CHU Rouen, Serv Chirurg & Digest, Rouen, France
[5] CHU Rouen, Serv Rhumatol, Rouen, France
[6] Univ Rouen, CNRS, UMR 6085, Rouen, France
[7] Univ Rouen, Lab Math Raphael Salem, Rouen, France
关键词
D O I
10.1002/hep.20052
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The goal of the current study was to provide complete coverage of the liver transcriptome with human probes corresponding to every gene expressed in embryonic, adult, and/or cancerous liver. We developed dedicated tools, namely, the Liverpool nylon array of complementary DNA (cDNA) probes for approximately 10,000 nonredundant genes and the Liver Tools database. Inflammation-induced transcriptome changes were studied in liver tissue samples from patients with an acute systemic inflammation and from control subjects. One hundred and fifty-four messenger RNAs (mRNA) correlated statistically with the extent of inflammation. Of these, 134 mRNA samples were not associated previously with an acute-phase (AP) response. The hepatocyte origin and proinflammatory cytokine responsiveness of these mRNAs were confirmed by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) in cytokine-challenged hepatoma cells. The corresponding gene promoters were enriched in potential binding sites for inflammation-driven transcription factors in the liver. Some of the corresponding proteins may provide novel blood markers of clinical relevance. The mRNAs whose level is most correlated with the AP extent (P < .05) were enriched in intracellular signaling molecules, transcription factors, glycosylation enzymes, and up-regulated plasma proteins. In conclusion, the hepatocyte responded to the AP extent by fine tuning some mRNA levels, controlling most, if not all, intracellular events from early signaling to the final secretion of proteins involved in innate immunity.
引用
收藏
页码:353 / 364
页数:12
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