Gene expression of oligodendrocyte markers in human amniotic epithelial cells using neural cell-type-specific expression system

被引:47
作者
Ishii, T
Ohsugi, K
Nakamura, S
Sato, K
Hashimoto, M
Mikoshiba, K
Sakuragawa, N
机构
[1] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Inherited Metab Dis, Kodaira, Tokyo 1808502, Japan
[2] Nihon Univ, Coll Bioresource Sci, Dept Appl Biol Sci, Fujisawa, Kanagawa 2520813, Japan
[3] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Biochem & Cellular Biol, Kodaira, Tokyo 1808502, Japan
[4] RIKEN, Inst Phys & Chem Res, Tsukuba Life Sci Ctr, Mol Neurobiol Lab, Tsukuba, Ibaraki 3050074, Japan
[5] Univ Tokyo, Inst Med Sci, Dept Mol Neurobiol, Tokyo 1080071, Japan
关键词
human amniotic epithelial cells; neural cell markers; gene expression; neural cell-type-specific expression system; myelin basic protein;
D O I
10.1016/S0304-3940(99)00297-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have previously reported that human amniotic epithelial (HAE) cells expressed neuronal and glial cell markers using immunostaining and western blotting. To study the expression system of these cell markers in HAE cells, we investigated the expression of mRNA for oligodendrocyte markers in HAE cells by reverse-transcriptase-polymerase chain reaction (RT-PCR) and northern blotting. Neural cell-type-specific expression system was used to examine the transcriptional activity of myelin basic protein (MBP). Oligodendrocyte markers were expressed such as CNPase, MBP and proteolipid protein (PLP and DM-20). PLP gene transcripts in the cells were in a lower level than DM-20, compared with those of human brain. Neural cell-type-specific expression system disclosed HAE cells were about 20% positive for beta-Gal using AdexMBP-NL-LacZ. This strongly indicates that HAE cells have MBP-specific gene expressing cells. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:131 / 134
页数:4
相关论文
共 17 条
  • [1] DEVELOPMENTAL REGULATION OF MYELIN BASIC-PROTEIN EXPRESSION IN MOUSE-BRAIN
    CARSON, JH
    NIELSON, ML
    BARBARESE, E
    [J]. DEVELOPMENTAL BIOLOGY, 1983, 96 (02) : 485 - 492
  • [2] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [3] Evidence for synthesis and release of catecholamines by human amniotic epithelial cells
    Elwan, MA
    Sakuragawa, N
    [J]. NEUROREPORT, 1997, 8 (16) : 3435 - 3438
  • [4] REDUCTION OF BACKGROUND PROBLEMS IN NONRADIOACTIVE NORTHERN AND SOUTHERN BLOT ANALYSES ENABLES HIGHER SENSITIVITY THAN P-32 BASED HYBRIDIZATIONS
    ENGLERBLUM, G
    MEIER, M
    FRANK, J
    MULLER, GA
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 210 (02) : 235 - 244
  • [5] GENETICALLY MODIFIED CELLS - APPLICATIONS FOR INTRACEREBRAL GRAFTING
    GAGE, FH
    KAWAJA, MD
    FISHER, LJ
    [J]. TRENDS IN NEUROSCIENCES, 1991, 14 (08) : 328 - 333
  • [6] A neural cell-type-specific expression system using recombinant adenovirus vectors
    Hashimoto, M
    Aruga, J
    Hosoya, Y
    Kanegae, Y
    Saito, I
    Mikoshiba, K
    [J]. HUMAN GENE THERAPY, 1996, 7 (02) : 149 - 158
  • [7] EXPRESSION OF MYELIN PROTEINS IN THE DEVELOPING HUMAN SPINAL-CORD - CLONING AND SEQUENCING OF HUMAN PROTEOLIPID PROTEIN CDNA
    KRONQUIST, KE
    CRANDALL, BF
    MACKLIN, WB
    CAMPAGNONI, AT
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1987, 18 (03) : 395 - 401
  • [8] CDNA CLONING AND AMINO-ACID SEQUENCE OF HUMAN-BRAIN 2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE
    KURIHARA, T
    TAKAHASHI, Y
    NISHIYAMA, A
    KUMANISHI, T
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (02) : 837 - 842
  • [9] CELL-SPECIFIC EXPRESSION OF THE MOUSE GLIAL FIBRILLARY ACIDIC PROTEIN GENE - IDENTIFICATION OF THE CIS-ACTING AND TRANS-ACTING PROMOTER ELEMENTS FOR ASTROCYTE-SPECIFIC EXPRESSION
    MIURA, M
    TAMURA, T
    MIKOSHIBA, K
    [J]. JOURNAL OF NEUROCHEMISTRY, 1990, 55 (04) : 1180 - 1188
  • [10] Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome
    Miyake, S
    Makimura, M
    Kanegae, Y
    Harada, S
    Sato, Y
    Takamori, K
    Tokuda, C
    Saito, I
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (03) : 1320 - 1324