Placenta-restricted expression of LTR-derived NOS3

被引：24

作者：

Huh, J. -W. ^{[1
,3
]}

Ha, H. -S. ^{[1
]}

Kim, D. -S. ^{[2
]}

Kim, H. -S. ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Div Biol Sci, Coll Nat Sci, Pusan 609735, South Korea

[2] Pusan Natl Univ, PBBRC, Interdisciplinary Res Program Bioinformat, Pusan 609735, South Korea

[3] KRIBB, NPRC, Ochang 363883, Chungbuk, South Korea

来源：

PLACENTA | 2008年 / 29卷 / 07期

关键词：

NOS3; LTR10A; placenta; methylation; reporter gene assay;

D O I：

10.1016/j.placenta.2008.04.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Domestication events of long terminal repeat (LTR) sequences of the human endogenous retrovirus (HERV) family have been considered to be a new mechanism for the generation of alternative splicing in the human genome. We investigated an LTR10A belonging to the HERV-1 family at the human endothelial nitric oxide synthase (NOS3) gene locus. The LTR10A element was located upstream of the original promoter sequences of NOS3. Expression analysis using RT-PCR and reporter gene assays in HCT116 and COS7 cells indicated placenta-specific expression of NOS3 driven by the LTR10A-derived promoter. The placenta-restricted expression was also determined to be associated with hypomethylation of the LTR10A element by methylation analysis using sodium bisulfite DNA sequencing. Furthermore, treatment of brain-derived cell lines with demethylation reagents did not restore expression of the LTR-derived NOS3 gene transcript. Taken together, the integration event of an LTR10A element in the upstream region of NOS3 led to the generation of a placenta-specific alternative transcript governed by cooperative mechanisms of epigenetic control (DNA methylation) and transcriptional regulation (interaction between cis- and trans-acting elements). (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：602 / 608

页数：7

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