Solitary HERV-K LTRs possess bi-directional promoter activity and contain a negative regulatory element in the U5 region

被引：100

作者：

Domansky, AN

Kopantzev, EP

Snezhkov, EV

Lebedev, YB

Leib-Mosch, C

Sverdlov, ED

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

[2] GSF Forschungszentrum Umwelt & Gesundheit, Inst Mol Virol, D-85758 Oberschleissheim, Germany

来源：

FEBS LETTERS | 2000年 / 472卷 / 2-3期

基金：

俄罗斯基础研究基金会;

关键词：

human endogenous retrovirus; HERV-K; long terminal repeat; regulation of transcription;

D O I：

10.1016/S0014-5793(00)01460-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene, Deletion analysis demonstrated the possible existence of alternative promoters within the LTR as well as a silencer-like element in the U5 region. Our results indicate also that all-trans-retinoic acid is capable of modulating expression of the reporter gene directed by a HERV-K LTR in NT2/D1 cells. (C) 2000 Federation of European Biochemical Societies.

引用

页码：191 / 195

页数：5

共 25 条

[1] Long terminal repeats of human endogenous retrovirus K family (HERV-K) specifically bind host cell nuclear proteins [J].