Measurement of pH Values in Human Tissues by Two-Photon Microscopy

被引：88

作者：

Park, Hee Jung ^{[1
]}

Lim, Chang Su ^{[1
]}

Kim, Eun Sun ^{[2
]}

Han, Ji Hee ^{[1
]}

Lee, Tae Hee ^{[3
]}

Chun, Hoon Jai ^{[2
]}

Cho, Bong Rae ^{[1
]}

机构：

[1] Korea Univ, Dept Chem, Seoul 136713, South Korea

[2] Korea Univ, Sch Med, Seoul 136713, South Korea

[3] Chungnam Natl Univ, Dept Chem, Taejon, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 11期

关键词：

fluorescent probes; imaging agents; live-cell imaging; naphthalenes; two-photon microscopy; GUIDELINES; DIAGNOSIS; PROBES;

D O I：

10.1002/anie.201109052

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

pH values go live! A ratiometric two-photon (TP) probe (NP1, see scheme) that has a significant TP action cross-section, high photostability, negligible toxicity, and can estimate pH values in live cells and human tissues by two-photon microscopy is described. NP1 can detect the difference in pH between live cells from the gastroesophageal junction (GEJ) and the lower esophageal sphincter of patients with and without esophagitis (see image). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2673 / 2676

页数：4

共 16 条

[1] Quantifying Colocalization by Correlation: The Pearson Correlation Coefficient is Superior to the Mander's Overlap Coefficient [J].