Determination of phenolic compounds in rose hip (Rosa canina) using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode-array detection

被引:195
作者
Hvattum, E [1 ]
机构
[1] Agr Univ Norway, Dept Chem & Biotechnol, N-1432 As, Norway
关键词
D O I
10.1002/rcm.622
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Liquid chromatography coupled with negative and positive electrospray ionisation (ESI) tandem mass spectrometry (MS/MS) and diode-array detection (DAD) was used for determination of phenols in rose hip (Rosa canina) extract. ESI mass spectra of the chromatographically separated phenols gave the molecular weight of the compounds through prominent [M-H](-) ions for most of the compounds and M+ ions for the anthocyanins. Collision induced dissociation (CID) of the [M-H](-) (or M+) precursor ions yielded product ions which determined the molecular weight of the aglycones. In-source fragmentation followed by CID of the resulting deprotonated aglycone ([A-H](-)) provided product ions for the identification of the unconjugated phenols. The identification was based on comparison with product ion spectra of commercial standards. UV-diode-array spectra were used for identity confirmation. This combined approach allowed the identification in rose hip extract of an anthocyanin, i.e. cyanidin-3-O-glucoside, several glycosides of quercetin and glycosides of taxifolin and eriodictyol. Phloridzin was identified, and several conjugates of methyl gallate were also found, one of which was tentatively identified as methyl gallate-rutinoside. Catechin and quercetin were found as the aglycones in the extract. Copyright (C) 2002 John Wiley Sons, Ltd.
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页码:655 / 662
页数:8
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