Electrochemical DNA biosensor as a screening tool for the detection of toxicants in water and wastewater samples

被引：93

作者：

Lucarelli, F ^{[1
]}

Palchetti, I ^{[1
]}

Marrazza, G ^{[1
]}

Mascini, M ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Chim, I-50019 Florence, Italy

来源：

TALANTA | 2002年 / 56卷 / 05期

关键词：

DNA biosensor; toxicity; screen-printed electrodes; Toxalert (R) 100;

D O I：

10.1016/S0039-9140(01)00655-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Applications of a disposable electrochemical DNA biosensor to standard solutions and to real samples are reported. The DNA biosensor is assembled by immobilising the double stranded calf thymus DNA on the surface of a disposable carbon screen-printed electrode. The immobilised ds-DNA interacts with the sample for 2 min; then is washed and immersed in a clean buffer where the analytical signal (the oxidation peak area of the guanine base) is obtained by a square-wave voltammetric scan. The results were compared with some currently used toxicity tests and in particular with a commercial luminescent bacteria test, Toxalert(R)100. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：949 / 957

页数：9

共 19 条

[1]

[Anonymous], 1999, REPUBBLICA ITALIANA

[2]

BARCELO D, 2000, BIOSET FINAL REPORT

[3] DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING [J].

CAGNINI, A ;

PALCHETTI, I ;

LIONTI, I ;

MASCINI, M ;

TURNER, APF .

SENSORS AND ACTUATORS B-CHEMICAL, 1995, 24 (1-3) :85-89

[4] Identification of polar toxicants in industrial wastewaters using toxicity-based fractionation with liquid chromatography/mass spectrometry [J].

Castillo, M ;

Barcelo, D .

ANALYTICAL CHEMISTRY, 1999, 71 (17) :3769-3776

[5] Characterisation of organic pollutants in textile wastewaters and landfill leachate by using toxicity-based fractionation methods followed by liquid and gas chromatography coupled to mass spectrometric detection [J].

Castillo, M ;

Barceló, D .

ANALYTICA CHIMICA ACTA, 2001, 426 (02) :253-264

[6] Electrochemical DNA biosensor for environmental monitoring [J].

Chiti, G ;

Marrazza, G ;

Mascini, M .

ANALYTICA CHIMICA ACTA, 2001, 427 (02) :155-164

[7]

FARRE M, 2001, ANAL CHIM ACTA, V427, P81

[8] A supercoiled DNA-modified mercury electrode-based biosensor for the detection of DNA strand cleaving agents [J].

Fojta, M ;

Stankova, V ;

Palecek, E ;

Koscielniak, P ;

Mitas, J .

TALANTA, 1998, 46 (01) :155-161

[9]

[Greenberg A.E. Standard Methods Standard Methods], 1998, STANDARD METHODS EXA, V20th

[10] Determination of anticholinesterase activity for pesticides monitoring using a thiocholine sensor [J].

Hernandez, S ;

Palchetti, I ;

Mascini, M .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 2000, 78 (3-4) :263-278

← 1 2 →