How to achieve rapid separations in counter-current chromatography

被引：20

作者：

Chen, LJ

Sutherland, IA ^{[1
]}

机构：

[1] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

[2] Sichuan Univ, W China Hosp, W China Med Sch, Div Canc Biotherapy,State Key Lab Biotherapy, Chengdu 610041, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1114卷 / 01期

关键词：

oroxylum indicum; high-speed counter-current chromatography (HSCCC); flavonoids;

D O I：

10.1016/j.chroma.2006.02.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new generation of high performance coil planet centrifuges is now making it possible to realize the enormous potential of liquid-liquid partition chromatography for the rapid and predictable scale up of separation processes. This paper uses a separation of flavonoids to demonstrate how rapid fractionations can be obtained at high flow rates with limited loss of resolution and with significant increase in throughput. Furthermore, it is shown that chromatograms at various flows can be modeled so that optimum conditions can be rapidly assessed. (c) 2006 Published by Elsevier B.V.

引用

页码：29 / 33

页数：5

共 14 条

[1]

Berthod A, 2002, COMP ANAL C, V38, P21, DOI 10.1016/S0166-526X(02)80005-8

[2] Modeling the performance of pilot-scale countercurrent chromatography: Scale-up predictions and experimental verification of erythromycin separation [J].