Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers

被引：64

作者：

Wu, Chen ^{[1
]}

Xie, Yuanyuan ^{[1
]}

Gao, Feng ^{[2
]}

Wang, Yanan ^{[3
]}

Guo, Yawei ^{[1
]}

Tian, Huanha ^{[1
]}

Li, Yanshuang ^{[1
]}

Fan, Wufang ^{[1
]}

机构：

[1] Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China

[2] Tradit Chinese Med Hosp Jiangsu Prov, Clin Lab, Nanjing 210029, Jiangsu, Peoples R China

[3] Hebei Univ, Dept Pathol, Affiliated Hosp, Baoding 071000, Hebei, Peoples R China

来源：

GENE | 2013年 / 525卷 / 01期

关键词：

Gastric adenocarcinoma; Cancer stem cells; Lgr5; Bmi1; Immunohistochemistry; ALDEHYDE DEHYDROGENASE 1; SELF-RENEWAL; GASTROINTESTINAL-TRACT; COLORECTAL-CANCER; BMI-1; TUMORS; IDENTIFICATION; EPIDEMIOLOGY; PROGENITOR; INTESTINE;

D O I：

10.1016/j.gene.2013.04.067

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units; and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26 +, CD44 +, ALDH1 + and CD133 + cells co-existed with Lgr5 + cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：18 / 25

页数：8

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