Decreased level of cyclin A2 in rat cochlea development and cochlear stem cell differentiation

被引：9

作者：

Chen, Jun ^{[1
]}

Wang, Feng ^{[1
]}

Gao, Xue ^{[1
]}

Zha, Dingjun ^{[1
]}

Xue, Tao ^{[1
]}

Cheng, Xin ^{[1
]}

Zhong, Cuiping ^{[1
]}

Han, Yu ^{[1
]}

Qiu, Jianhua ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Otolaryngol, Xian 710032, Shaanxi Prov, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2009年 / 453卷 / 03期

关键词：

Inner ear; Cochlea; Cyclin A2; Stem cells; Rat; HAIR-CELLS; PROLIFERATION; GROWTH; GENES; ORGAN;

D O I：

10.1016/j.neulet.2009.02.019

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cyclin A2 plays a major role in cell cycle modulation. Cyclin A2 level has not been determined in the inner ear yet. RT-PCR, Western blotting, immunohistochemistry and immunocytochemistry were used to measure cyclin A2 expression in rat cochlea tissues of different ages, isolated cochlear stem cells and stem cell-derived differentiated cells. The results indicated that cyclin A2 level in cochlea tissues decreased gradually from newborn to adult. Furthermore, cyclin A2 level fell down after differentiation of cochlear stem cells. It was suggested that cyclin A2 might be involved in the modulation of rat cochlea development and cochlear stem cell differentiation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：166 / 169

页数：4

共 17 条

[1] Cyclin A2 mediates cardiomyocyte mitosis in the postmitotic myocardium [J].

Chaudhry, HW ;

Dashoush, NH ;

Tang, HY ;

Zhang, L ;

Wang, XY ;

Wu, EX ;

Wolgemuth, DJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (34) :35858-35866

[2] Progressive hearing loss in mice lacking the cyclin-dependent kinase inhibitor Ink4d [J].

Chen, P ;

Zindy, F ;

Abdala, C ;

Liu, F ;

Li, XK ;

Roussel, MF ;

Segil, N .

NATURE CELL BIOLOGY, 2003, 5 (05) :422-426

[3] Role of the F-box protein Skp2 in cell proliferation in the developing auditory system in mice [J].

Dong, YY ;

Nakagawa, T ;

Endo, T ;

Kim, TS ;

Iguchi, F ;

Yamamoto, N ;

Naito, Y ;

Ito, J .

NEUROREPORT, 2003, 14 (05) :759-761

[4]

GRANA X, 1995, ONCOGENE, V11, P211

[5] P27Kip1 deficiency causes organ of Corti pathology and hearing loss [J].

Kanzaki, Sho ;

Beyer, Lisa A. ;

Swiderski, Donald L. ;

Izumikawa, Masahiko ;

Stover, Timo ;

Kawamoto, Kohei ;

Raphael, Yehoash .

HEARING RESEARCH, 2006, 214 (1-2) :28-36

[6] Pluripotent stem cells from the adult mouse inner ear [J].

Li, HW ;

Liu, H ;

Heller, S .

NATURE MEDICINE, 2003, 9 (10) :1293-1299

[7] Cell cycle regulation in hair cell development and regeneration in the mouse cochlea [J].

Liu, Zhiyong ;

Zuo, Jian .

CELL CYCLE, 2008, 7 (14) :2129-2133

[8] Multipotent stem cells from the young rat inner ear [J].

Lou, Xiangxin ;

Zhang, Yan ;

Yuan, Chonggang .

NEUROSCIENCE LETTERS, 2007, 416 (01) :28-33

[9] Gene disruption of p27Kip1 allows cell proliferation in the postnatal and adult organ of Corti [J].

Löwenheim, H ;

Furness, DN ;

Ki, J ;

Zinn, C ;

Gültig, K ;

Fero, ML ;

Frost, D ;

Gummer, AW ;

Roberts, JM ;

Rubel, EW ;

Hackney, CM ;

Zenner, HP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :4084-4088

[10] Proliferative generation of mammalian auditory hair cells in culture [J].

Malgrange, B ;

Belachew, S ;

Thiry, M ;

Nguyen, L ;

Rogister, B ;

Alvarez, ML ;

Rigo, JM ;

Van de Water, TR ;

Moonen, G ;

Lefebvre, PP .

MECHANISMS OF DEVELOPMENT, 2002, 112 (1-2) :79-88

← 1 2 →