Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts

被引：15

作者：

Kullmann, Frank ^{[1
]}

Judex, Martin ^{[1
]}

Ballhorn, Wibke ^{[1
]}

Juesten, Hans-Peter ^{[2
]}

Wessinghage, Dieter ^{[2
]}

Welsh, John ^{[3
]}

Yen, Tim J. ^{[4
]}

Lang, Bernhard ^{[1
]}

Hittle, Jim C. ^{[4
]}

McClelland, Michael ^{[3
]}

Gay, Steffen ^{[5
]}

Schoelmerich, Juergen ^{[1
]}

Mueller-Ladner, Ulf ^{[1
]}

机构：

[1] Univ Regensburg, Dept Internal Med 1, D-93042 Regensburg, Bavaria, Germany

[2] Univ Regensburg, Dept Orthoped, D-93042 Regensburg, Bavaria, Germany

[3] Sidney Kimmel Canc Ctr, San Diego, CA USA

[4] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA

[5] Univ Hosp, Ctr Expt Rheumatol, Dept Rheumatol, Zurich, Switzerland

来源：

ARTHRITIS RESEARCH | 1999年 / 1卷 / 01期

关键词：

arthritis; centromere; differential display; immunohistochemistry; in situ hybridization; RNA fingerprinting;

D O I：

10.1186/ar13

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA.

引用

页码：71 / 80

页数：10

共 39 条

[11] ISOLATION OF A RECOMBINANT COPY OF THE GENE ENCODING C/EBP
LANDSCHULZ, WH
JOHNSON, PF
ADASHI, EY
GRAVES, BJ
MCKNIGHT, SL
[J]. GENES & DEVELOPMENT, 1988, 2 (07) : 786 - 800
[12] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION
LIANG, P
PARDEE, AB
[J]. SCIENCE, 1992, 257 (5072) : 967 - 971
[13] LIANG P, 1992, CANCER RES, V52, P6966
[14] Liang P, 1997, METHODS MOL BIOL, V87
[15] ANTIBODIES TO THE KINESIN MOTOR DOMAIN AND CENP-E INHIBIT MICROTUBULE DEPOLYMERIZATION-DEPENDENT MOTION OF CHROMOSOMES IN-VITRO
LOMBILLO, VA
NISLOW, C
YEN, TJ
GELFAND, VI
MCINTOSH, JR
[J]. JOURNAL OF CELL BIOLOGY, 1995, 128 (1-2) : 107 - 115
[16] Identification of human semaphorin E gene expression in rheumatoid synovial cells by mRNA differential display
MangasserStephan, K
Dooley, S
Welter, C
Mutschler, W
Hanselmann, RG
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 234 (01) : 153 - 156
[17] Maniatis T, 1997, MOL CLONING PROTOCOL
[18] Screening of differentially amplified cDNA products from RNA arbitrarily primed PCR fingerprints using single strand conformation polymorphism (SSCP) gels
MathieuDaude, F
Cheng, R
Welsh, J
McClelland, M
[J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (08) : 1504 - 1507
[19] RNA FINGERPRINTING AND DIFFERENTIAL DISPLAY USING ARBITRARILY PRIMED PCR
MCCLELLAND, M
MATHIEUDAUDE, F
WELSH, J
[J]. TRENDS IN GENETICS, 1995, 11 (06) : 242 - 246
[20] PROLIFERATION OF SYNOVIAL LINING CELLS AND FIBROBLASTS
MOHR, W
BENEKE, G
MOHING, W
[J]. ANNALS OF THE RHEUMATIC DISEASES, 1975, 34 (03) : 219 - 224

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