Staurosporine and herbimycin A augment agonist-induced elevation of cAMP in Jurkat T-lymphoblasts

被引:5
作者
Buc, HA [1 ]
Moncion, A [1 ]
Perignon, JL [1 ]
机构
[1] UNIV PARIS 05, FAC MED NECKER ENFANTS MALAD, F-75742 PARIS 15, FRANCE
来源
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 1996年 / 18卷 / 02期
关键词
human T-lymphocyte; prostaglandin E2; adenylyl cyclase; protein tyrosine kinase; staurosporine;
D O I
10.1016/0192-0561(95)00116-6
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cross-talk between signalling pathways appears to play an important role in T-lymphocyte activation. In the present work, we have studied the effects of different inhibitors of protein tyrosine kinases or protein serine/threonine kinases on the agonist-induced cAMP accumulation in the human T-lymphoblast cell line Jurkat. Staurosporine, a potent but nonspecific inhibitor of protein kinases, produced a ten-fold enhancement of the response to PGE2. No significant effect was obtained with two specific protein kinase C inhibitors (GF 109203X and H7), whereas herbimycin A, a specific protein tyrosine kinase inhibitor, markedly enhanced the PGE2-induced cAMP accumulation: its effect was approximately 60% that of staurosporine. It was confirmed that both staurosporine and herbimycin A inhibited by more than 90% the release of IP3 induced by ligation of the T-cell receptor, a known protein tyrosine kinase-dependent mechanism. To our knowledge, this study provides the first indication of a protein tyrosine kinase-mediated inhibition of agonist-induced cAMP accumulation. The possible targets of this inhibition are discussed. Copyright (C) 1996 International Society for Immunopharmacology
引用
收藏
页码:135 / 139
页数:5
相关论文
共 18 条
[1]   SIGNAL TRANSDUCTION THROUGH THE T-CELL ANTIGEN RECEPTOR [J].
ABRAHAM, RT ;
KARNITZ, LM ;
SECRIST, JP ;
LEIBSON, PJ .
TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (10) :434-438
[2]   DECREASE IN COUPLING OF GS IN V-SRC-TRANSFORMED NIH-3T3 FIBROBLASTS - POSSIBLE INVOLVEMENT OF TYROSINE PHOSPHORYLATION OF GS BY PP60(V-SRC) [J].
AKIHO, H ;
TOKUMITSU, Y ;
NODA, M ;
NOMURA, Y .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 304 (01) :235-241
[3]   ACTIVATION OF THE CD3/T-CELL RECEPTOR (TCR) COMPLEX OR OF PROTEIN-KINASE-C POTENTIATE ADENYLYL CYCLASE STIMULATION IN A TUMORAL T-CELL LINE - INVOLVEMENT OF 2 DISTINCT INTRACELLULAR PATHWAYS [J].
BIHOREAU, C ;
HEURTIER, A ;
ENJALBERT, A ;
CORVAIA, N ;
BENSUSSAN, A ;
DEGOS, L ;
KORDON, C .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (11) :2877-2882
[4]   T-CELL ANTIGEN RECEPTOR-MEDIATED ENHANCEMENT OF THE ADENYLATE-CYCLASE PATHWAY DEPENDS ON TYROSINE PROTEIN-KINASES [J].
BUC, HA ;
MONCION, A ;
HAMET, M ;
PERIGNON, JL .
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1993, 15 (03) :415-&
[5]   THE PROTEIN TYROSINE KINASE INHIBITOR HERBIMYCIN-A, BUT NOT GENISTEIN, SPECIFICALLY INHIBITS SIGNAL TRANSDUCTION BY THE T-CELL ANTIGEN RECEPTOR [J].
GRABER, M ;
JUNE, CH ;
SAMELSON, LE ;
WEISS, A .
INTERNATIONAL IMMUNOLOGY, 1992, 4 (11) :1201-1210
[6]   TYROSINE KINASE-STIMULATED GUANINE-NUCLEOTIDE EXCHANGE ACTIVITY OF VAV IN T-CELL ACTIVATION [J].
GULBINS, E ;
COGGESHALL, KM ;
BAIER, G ;
KATZAV, S ;
BURN, P ;
ALTMAN, A .
SCIENCE, 1993, 260 (5109) :822-825
[7]   GF-109203X, A SPECIFIC PKC INHIBITOR, ABROGATES ANTI-CD3 ANTIBODY-INDUCED UP-REGULATION OF CD4+ T-CELL ADHESION TO B-CELLS [J].
HAUSS, P ;
MAZEROLLES, F ;
HIVROZ, C ;
LECOMTE, O ;
BARBAT, C ;
FISCHER, A .
CELLULAR IMMUNOLOGY, 1993, 150 (02) :439-446
[8]  
INGLESE J, 1993, J BIOL CHEM, V268, P23735
[9]  
JUNE CH, 1990, J IMMUNOL, V144, P1591
[10]   THE ADENYLATE CYCLASE-CAMP-PROTEIN KINASE A PATHWAY AND REGULATION OF THE IMMUNE-RESPONSE [J].
KAMMER, GM .
IMMUNOLOGY TODAY, 1988, 9 (7-8) :222-229