Protochlorophyllide a: A Comprehensive Photophysical Picture

被引：48

作者：

Dietzek, Benjamin ^{[1
,2
]}

Tschierlei, Stefanie ^{[1
]}

Hermann, Gudrun ^{[3
]}

Yartsev, Arkady ^{[4
]}

Pascher, Torbjoern ^{[4
]}

Sundstrom, Villy ^{[4
]}

Schmitt, Michael ^{[1
]}

Popp, Juergen ^{[1
,2
]}

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Inst Photon Technol, D-07702 Jena, Germany

[3] Univ Jena, Inst Biochem & Biophys, D-07743 Jena, Germany

[4] Lund Univ, Dept Chem Phys, S-22100 Lund, Sweden

来源：

CHEMPHYSCHEM | 2009年 / 10卷 / 01期

关键词：

enzyme catalysis; photophysics; porphyrins; transient absorption spectroscopy; UV/Vis spectroscopy; TIME-RESOLVED ABSORPTION; EXCITED-STATE PROCESSES; CHLOROPHYLL BIOSYNTHESIS; ZINC TETRAPHENYLPORPHINE; ULTRAFAST DYNAMICS; OXIDOREDUCTASE; SPECTROSCOPY; DEPENDENCE; EVOLUTION; PERIDININ;

D O I：

10.1002/cphc.200800536

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed.

引用

页码：144 / 150

页数：7

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