Subdiffusion Supports Joining Of Correct Ends During Repair Of DNA Double-Strand Breaks

被引：35

作者：

Girst, S. ^{[1
]}

Hable, V. ^{[1
]}

Drexler, G. A. ^{[2
]}

Greubel, C. ^{[1
]}

Siebenwirth, C. ^{[1
]}

Haum, M. ^{[1
]}

Friedl, A. A. ^{[2
]}

Dollinger, G. ^{[1
]}

机构：

[1] Univ Bundeswehr Munchen, Angewandte Phys & Messtechn LRT2, D-85577 Neubiberg, Germany

[2] Univ Munich, Dept Radiat Oncol, D-80336 Munich, Germany

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

ANOMALOUS DIFFUSION; DYNAMICS; CELL; MOBILITY; PROTEINS; MOTION; SITES;

D O I：

10.1038/srep02511

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The mobility of damaged chromatin regions in the nucleus may affect the probability of mis-repair. In this work, live-cell observation and distance tracking of GFP-tagged DNA damage response protein MDC1 was used to study the random-walk behaviour of chromatin domains containing radiation-induced DNA double-strand breaks (DSB). Our measurements indicate a subdiffusion-type random walk process with similar time dependence for isolated and clustered DSBs that were induced by 20 MeV proton or 43 MeV carbon ion micro-irradiation. As compared to normal diffusion, subdiffusion enhances the probability that both ends of a DSB meet, thus promoting high efficiency DNA repair. It also limits their probability of long-range movements and thus lowers the probability of mis-rejoining and chromosome aberrations.

引用

页数：6

共 39 条

[1]

Nuclear organization and chromatin dynamics in yeast: Biophysical models or biologically driven interactions? [J].

Albert, Benjamin ;

Leger-Silvestre, Isabelle ;

Normand, Christophe ;

Gadal, Olivier .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2012, 1819 (06) :468-481

[2]

Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains [J].

Aten, JA ;

Stap, J ;

Krawczyk, PM ;

van Oven, CH ;

Hoebe, RA ;

Essers, J ;

Kanaar, R .

SCIENCE, 2004, 303 (5654) :92-95

[3]

Anomalous diffusion of proteins due to molecular crowding [J].

Banks, DS ;

Fradin, C .

BIOPHYSICAL JOURNAL, 2005, 89 (05) :2960-2971

[4]

Transient Anomalous Diffusion of Telomeres in the Nucleus of Mammalian Cells [J].

Bronstein, I. ;

Israel, Y. ;

Kepten, E. ;

Mai, S. ;

Shav-Tal, Y. ;

Barkai, E. ;

Garini, Y. .

PHYSICAL REVIEW LETTERS, 2009, 103 (01)

[5]

SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope [J].

Cabal, Ghislain G. ;

Genovesio, Auguste ;

Rodriguez-Navarro, Susana ;

Zimmer, Christophe ;

Gadal, Olivier ;

Lesne, Annick ;

Buc, Henri ;

Feuerbach-Fournier, Frank ;

Olivo-Marin, Jean-Christophe ;

Hurt, Eduard C. ;

Nehrbass, Ulf .

NATURE, 2006, 441 (7094) :770-773

[6]

Chromatin motion is constrained by association with nuclear compartments in human cells [J].

Chubb, JR ;

Boyle, S ;

Perry, P ;

Bickmore, WA .

CURRENT BIOLOGY, 2002, 12 (06) :439-445

[7]

Image-based modeling reveals dynamic redistribution of DNA damage into nuclear sub-domains [J].

Costes, Sylvain V. ;

Ponomarev, Artem ;

Chen, James L. ;

Nguyen, David ;

Cucinotta, Francis A. ;

Barcellos-Hoff, Mary Helen .

PLOS COMPUTATIONAL BIOLOGY, 2007, 3 (08) :1477-1488

[8]

The Munich microprobe SNAKE:: First results using 20 MeV protons and 90 MeV sulfur ions [J].

Datzmann, G ;

Dollinger, G ;

Goeden, C ;

Hauptner, A ;

Körner, HJ ;

Reichart, P ;

Schmelmer, O .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2001, 181 :20-26

[9]

Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy [J].

De Vos, W. H. ;

Joss, G. H. ;

Haffmans, W. ;

Hoebe, R. A. ;

Manders, E. M. M. ;

Van Oostveldt, P. .

JOURNAL OF MICROSCOPY, 2010, 238 (03) :254-264

[10]

Spatial Dynamics of DNA Damage Response Protein Foci along the Ion Trajectory of High-LET Particles [J].

Du, Guanghua ;

Drexler, Guido A. ;

Friedland, Werner ;

Greubel, Christoph ;

Hable, Volker ;

Kruecken, Reiner ;

Kugler, Alexandra ;

Tonelli, Laura ;

Friedl, Anna A. ;

Dollinger, Guenther .

RADIATION RESEARCH, 2011, 176 (06) :706-715

← 1 2 3 4 →