Transient Anomalous Diffusion of Telomeres in the Nucleus of Mammalian Cells

被引：413

作者：

Bronstein, I. ^{[1
,2
]}

Israel, Y. ^{[1
,2
]}

Kepten, E. ^{[1
,2
]}

Mai, S. ^{[3
]}

Shav-Tal, Y. ^{[2
,4
]}

Barkai, E. ^{[1
,2
]}

Garini, Y. ^{[1
,2
,5
]}

机构：

[1] Bar Ilan Univ, Dept Phys, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Inst Nanotechnol, IL-52900 Ramat Gan, Israel

[3] Univ Manitoba, CancerCare Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada

[4] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel

[5] Delft Univ Technol, Dept Imaging Sci & Technol, NL-2628 CJ Delft, Netherlands

来源：

PHYSICAL REVIEW LETTERS | 2009年 / 103卷 / 01期

基金：

以色列科学基金会;

关键词：

DYNAMICS; CHROMATIN; LANDSCAPE; GENOME;

D O I：

10.1103/PhysRevLett.103.018102

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We measured individual trajectories of fluorescently labeled telomeres in the nucleus of eukaryotic cells in the time range of 10(-2)-10(4)sec by combining a few acquisition methods. At short times the motion is subdiffusive with << r(2)>>similar to t(alpha) and it changes to normal diffusion at longer times. The short times diffusion may be explained by the reptation model and the transient diffusion is consistent with a model of telomeres that are subject to a local binding mechanism with a wide but finite distribution of waiting times. These findings have important biological implications with respect to the genome organization in the nucleus.

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