Transient Anomalous Diffusion of Telomeres in the Nucleus of Mammalian Cells

被引:413
作者
Bronstein, I. [1 ,2 ]
Israel, Y. [1 ,2 ]
Kepten, E. [1 ,2 ]
Mai, S. [3 ]
Shav-Tal, Y. [2 ,4 ]
Barkai, E. [1 ,2 ]
Garini, Y. [1 ,2 ,5 ]
机构
[1] Bar Ilan Univ, Dept Phys, IL-52900 Ramat Gan, Israel
[2] Bar Ilan Univ, Inst Nanotechnol, IL-52900 Ramat Gan, Israel
[3] Univ Manitoba, CancerCare Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada
[4] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-52900 Ramat Gan, Israel
[5] Delft Univ Technol, Dept Imaging Sci & Technol, NL-2628 CJ Delft, Netherlands
基金
以色列科学基金会;
关键词
DYNAMICS; CHROMATIN; LANDSCAPE; GENOME;
D O I
10.1103/PhysRevLett.103.018102
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We measured individual trajectories of fluorescently labeled telomeres in the nucleus of eukaryotic cells in the time range of 10(-2)-10(4)sec by combining a few acquisition methods. At short times the motion is subdiffusive with << r(2)>>similar to t(alpha) and it changes to normal diffusion at longer times. The short times diffusion may be explained by the reptation model and the transient diffusion is consistent with a model of telomeres that are subject to a local binding mechanism with a wide but finite distribution of waiting times. These findings have important biological implications with respect to the genome organization in the nucleus.
引用
收藏
页数:4
相关论文
共 24 条
[11]   Random time-scale invariant diffusion and transport coefficients [J].
He, Y. ;
Burov, S. ;
Metzler, R. ;
Barkai, E. .
PHYSICAL REVIEW LETTERS, 2008, 101 (05)
[12]   Nonergodicity mimics inhomogeneity in single particle tracking [J].
Lubelski, Ariel ;
Sokolov, Igor M. ;
Klafter, Joseph .
PHYSICAL REVIEW LETTERS, 2008, 100 (25)
[13]   The random walk's guide to anomalous diffusion: a fractional dynamics approach [J].
Metzler, R ;
Klafter, J .
PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS, 2000, 339 (01) :1-77
[14]   Visualizing telomere dynamics in living mammalian cells using PNA probes [J].
Molenaar, C ;
Wiesmeijer, K ;
Verwoerd, NP ;
Khazen, S ;
Eils, R ;
Tanke, HJ ;
Dirks, RW .
EMBO JOURNAL, 2003, 22 (24) :6631-6641
[15]   Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques [J].
Murase, K ;
Fujiwara, T ;
Umemura, Y ;
Suzuki, K ;
Iino, R ;
Yamashita, H ;
Saito, M ;
Murakoshi, H ;
Ritchie, K ;
Kusumi, A .
BIOPHYSICAL JOURNAL, 2004, 86 (06) :4075-4093
[16]   Regulation of telomere movement by telomere chromatin structure [J].
Pandita T.K. ;
Hunt C.R. ;
Sharma G.G. ;
Yang Q. .
Cellular and Molecular Life Sciences, 2007, 64 (2) :131-138
[17]   Global nature of dynamic protein-chromatin interactions in vivo:: Three-dimensional genome scanning and dynamic interaction networks of chromatin proteins [J].
Phair, RD ;
Scaffidi, P ;
Elbi, C ;
Vecerová, J ;
Dey, A ;
Ozato, K ;
Brown, DT ;
Hager, G ;
Bustin, M ;
Misteli, T .
MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (14) :6393-6402
[18]   The biological frontier of physics [J].
Phillips, R ;
Quake, SR .
PHYSICS TODAY, 2006, 59 (05) :38-43
[19]   Cajal Body dynamics and association with chromatin are ATP-dependent [J].
Platani, M ;
Goldberg, I ;
Lamond, AI ;
Swedlow, JR .
NATURE CELL BIOLOGY, 2002, 4 (07) :502-508
[20]   A biological interpretation of transient anomalous subdiffusion. I. Qualitative model [J].
Saxton, Michael J. .
BIOPHYSICAL JOURNAL, 2007, 92 (04) :1178-1191