High-resolution NMR "chromatography" using a liquids spectrometer

被引:59
作者
Hoffman, Roy E. [1 ]
Arzuan, Hilla [2 ]
Pemberton, Chava [2 ]
Aserin, Abraham [2 ]
Garti, Nissim [2 ]
机构
[1] Hebrew Univ Jerusalem, Dept Organ Chem, IL-91904 Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Inst Chem, Casali Inst Appl Chem, IL-91904 Jerusalem, Israel
关键词
Diffusion; NMR chromatography; DOSY; Magnetic susceptibility; Silica;
D O I
10.1016/j.jmr.2008.06.022
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
NMR spectroscopy is an excellent tool for the structural analysis of pure compounds. However, for mixtures it performs poorly because of overlapping signals. Diffusion can be used to separate compounds of widely differing molecular weight but the amount of separation is usually insufficient. Addition of a solid medium, analogous to the stationary phase in chromatography, can preferentially slow the diffusion of some components of a mixture permitting separation in the diffusion dimension. However, this would usually require a solid-state NMR spectrometer otherwise the signals would be too broad. Susceptibility matching the solvent to the solid medium yields a spectrum with narrow signals allowing the measurement of a DOSY spectrum with enhanced separation in the diffusion dimension. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:295 / 299
页数:5
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