Taxol-induced apoptotic cell death in suspension cultures of Taxus cuspidata

被引：6

作者：

Yuan, YJ ^{[1
]}

Ma, ZY ^{[1
]}

Wu, JC ^{[1
]}

Zeng, AP ^{[1
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Dept Pharmaceut Engn, Tianjin 300072, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 08期

基金：

中国国家自然科学基金;

关键词：

apoptosis; DNA fragment; suspension culture; Taxol; Taxus cuspidata;

D O I：

10.1023/A:1015062405020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Taxol caused apoptotic cell death of Taxus cuspidata in suspension cultures. Typical morphological and biochemical changes of apoptosis were observed by microscopy and total DNA agarose gel electrophoresis. Taxus cuspidata responded to the added Taxol by increasing the biosynthesis of Taxol. The percentage of apoptotic cells in total cells increased with the concentration of added Taxol. With Taxol added at 10 mg l(-1), the maximum concentration of Taxol produced was 23 mg l(-1), 3 times higher than that of the control culture.

引用

页码：615 / 618

页数：4

共 14 条

[1] Plasma membrane-associated actin in bright yellow 2 tobacco cells - Evidence for interaction with microtubules [J].

Collings, DA ;

Asada, T ;

Allen, NS ;

Shibaoka, H .

PLANT PHYSIOLOGY, 1998, 118 (03) :917-928

[2]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

[3]

HOROWITZ SB, 1986, ANN NY ACAD SCI, V466, P733

[4] MECHANISM OF MITOTIC BLOCK AND INHIBITION OF CELL-PROLIFERATION BY TAXOL AT LOW CONCENTRATIONS [J].

JORDAN, MA ;

TOSO, RJ ;

THROWER, D ;

WILSON, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9552-9556

[5]

Ketchum REB, 1999, BIOTECHNOL BIOENG, V62, P97, DOI 10.1002/(SICI)1097-0290(19990105)62:1&lt

[6]

97::AID-BIT11&gt

[7]

3.0.CO

[8]

2-C

[9] Apoptotic cell death in suspension cultures of Taxus chinensis var. mairei [J].

Ma, ZY ;

Yuan, YJ ;

Wu, JC ;

Zeng, AP .

BIOTECHNOLOGY LETTERS, 2002, 24 (07) :573-577

[10]

SVOANOVSKY SR, 1999, J PHARM BIOMED ANAL, V20, P3549

← 1 2 →