MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2

被引：56

作者：

Gao, Feng ^{[1
]}

Wu, Hao ^{[2
]}

Wang, Rui ^{[1
]}

Guo, Yang ^{[1
]}

Zhang, Zefeng ^{[1
]}

Wang, Tao ^{[1
]}

Zhang, Guoliang ^{[1
]}

Liu, Changjiang ^{[1
]}

Liu, Junfeng ^{[1
]}

机构：

[1] Hebei Med Univ, Dept Thorac Surg, Hosp 4, 12 Jiankang Rd, Shijiazhuang 050011, Hebei, Peoples R China

[2] Hebei Med Univ, Dept Clin Lab, Shijiazhuang, Hebei, Peoples R China

来源：

BIOENGINEERED | 2019年 / 10卷 / 01期

关键词：

miR-485-5p; FLOT2; small cell lung cancer; proliferation; metastasis; LIPID RAFT; OSTEOSARCOMA; MICRODOMAINS; GROWTH; METASTASIS; MECHANISM;

D O I：

10.1080/21655979.2019.1586056

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

This study is aimed to elucidate the mechanisms underlying the role of miR-485-5p in small cell lung cancer (SCLC). The expression of miR-485-5p were quantified with real time quantitative PCR and it was found that the level of miR-485-5p was lower in SCLC tissues than normal tissues. In cultured SCLC cell lines, overexpression of miR-485-5p reduced cell proliferation, migration, and invasion in vitro, whereas knockdown of miR-485-5p performed contrary. FLOT2 expression was obviously upregulated and negatively correlated with miR-485-5p expression level in SCLC tissues. Overexpression of miR-485-5p significantly inhibited the protein expression of flotillin-2 (FLOT2) in cultured SCLC cells. Luciferase reporter assay confirmed that FLOT2 was a direct target of miR-485-5p in SCLC cells. It is concluded that miR-485-5p, as a tumor suppressor, inhibits the growth and metastasis in SCLC by targeting FLOT2. Upregulation of miR-485-5p expression may be an attractive strategy for SCLC therapy.

引用

页码：1 / 12

页数：12

共 38 条

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