Differential effects of fenpropimorph and fenhexamid, two sterol biosynthesis inhibitor fungicides, on arbuscular mycorrhizal development and sterol metabolism in carrot roots

被引：33

作者：

Campagnac, Estelle ^{[1
]}

Fontaine, Joel ^{[1
]}

Sahraoui, Anissa Lounes-Hadj ^{[1
]}

Laruelle, Frederic ^{[1
]}

Durand, Roger ^{[1
]}

Grandmougin-Ferjani, Anne ^{[1
]}

机构：

[1] Univ Littoral Cote dOpale, Lab Mycol Phytopathol Environm, F-62228 Calais, France

来源：

PHYTOCHEMISTRY | 2008年 / 69卷 / 17期

关键词：

Daucus carota; Apiaceae; Glomus intraradices; Glomeraceae; Arbuscular mycorrhizae; Symbiosis; Lipid; Fungicide; Sterol biosynthesis inhibitor (SBI); Monoxenic cultures;

D O I：

10.1016/j.phytochem.2008.09.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sterols composition of transformed carrot roots incubated in presence of increasing concentrations of fenpropimorph (0.02; 0.2; 2 mg l(-1)) and fenhexamid (0.02; 0.2; 2; 20 mg l(-1)), colonized or not by Glomus intraradices was determined. In mycorrhizal roots treated with fenpropimorph, normal Delta(5)-sterols were replaced by unusual compounds such as 9 beta, 19-cyclopropylsterols (24-methylpollinastanol), Delta(8,14)-sterols (ergosta-8,14-dienol, stigmasta-8,14-dienol), Delta(8)-sterols (Delta(8) sitosterol) and Delta(7)-sterols (ergosta-7,22-dienol). After application of fenpropimorph, a drastic reduction of the mycorrhizal root growth, root colonization and extraradical fungal development was observed. Application of fenhexamid did not modify sterol profiles and the total colonization of roots. But the arbuscule frequency of the fungal partner was significantly affected. Comparison of the effects caused by the tested fungicides indicates that the usual phytosterols may be involved in symbiosis development. Indeed, observed modifications of root sterols composition could explain the high fenpropimorph toxicity to the AM symbiosis. However, the absence of sterolic modifications in the roots treated with fenhexamid could account for its more limited impact on mycorrhization. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2912 / 2919

页数：8