Differentiation of the volatile profile of microbiologically contaminated canned tomatoes by dynamic headspace extraction followed by gas chromatography-mass spectrometry analysis

被引:37
作者
Bianchi, F. [1 ]
Careri, M. [1 ]
Mangia, A. [1 ]
Mattarozzi, M. [1 ]
Musci, M. [1 ]
Concina, I. [2 ,3 ]
Falasconi, M. [2 ]
Gobbi, E. [4 ]
Pardo, M. [2 ]
Sberveglieri, G. [2 ]
机构
[1] Univ Parma, Dipartimento Chim Gen & Inorgan, I-43100 Parma, Italy
[2] Univ Brescia, Dipartimento Chim & Fis Ingn & Mat, INFM, CNR,SENSOR Lab, I-25133 Brescia, Italy
[3] CNR, Ist Biofis, I-90146 Palermo, Italy
[4] Univ Udine, DBADP, I-33100 Udine, Italy
关键词
Tomato; Dynamic headspace; Mass spectrometry; Aromatic profile; Microbial contamination; SOLID-PHASE MICROEXTRACTION; ESCHERICHIA-COLI O157-H7; FRESH TOMATO; ELECTRONIC NOSE; SPOILAGE BACTERIA; AROMA; PRODUCTS; QUALITY; SYSTEM; AUTHENTICATION;
D O I
10.1016/j.talanta.2008.07.061
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The aromatic profile of microbiologically contaminated canned tomatoes was analyzed by the dynamic headspace extraction technique coupled with gas chromatography-mass spectrometry. Canned tomatoes contaminated with Escherichia coli, Saccharomyces cerevisiae and Aspergillus carbonarius were analyzed after 2 and 7 days. About 100 volatiles were detected, among which alcohols, aldehydes and ketones were the most abundant compounds. Gas chromatographic peak areas were used for statistical purposes. First, principal component analysis was carried out in order to visualize data trends and clusters. Then, linear discriminant analysis was performed in order to detect the set of volatile compounds ables to differentiate groups of analyzed samples. Five volatile compounds, i.e. ethanol, beta-myrcene,o-methyl styrene, 6-methyl-5-hepten-2-ol and 1-octanol, were found to be able to better discriminate between uncontaminated and contaminated samples. Prediction ability of the calculated model was estimated to be 100% by the "leave-one-out" cross-validation. An electronic nose device was then used to analyze the same contaminated and not contaminated canned tomato samples. Preliminary results were compared with those obtained by dynamic headspace gas chromatography-mass spectrometry, showing a good agreement. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:962 / 970
页数:9
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