Evidence that the BBA68 protein (BbCRASP-1) of the Lyme disease Spirochetes does not contribute to factor H-mediated immune evasion in humans and other animals

被引：46

作者：

McDowell, JV

Hovis, KM

Zhang, HM

Tran, E

Lankford, J

Marconi, RT

机构：

[1] Virginia Commonwealth Univ, Med Coll Virginia, Dept Immunol & Microbiol, Richmond, VA 23298 USA

[2] Virginia Commonwealth Univ, Med Coll Virginia, Ctr Study Biol Complex, Richmond, VA 23298 USA

来源：

INFECTION AND IMMUNITY | 2006年 / 74卷 / 05期

关键词：

D O I：

10.1128/IAI.74.5.3030-3034.2006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

BBA68 (BbCRASP-1) of the Lyme disease spirochetes binds human factor H (FH) and FH-like protein 1. (FHL-1). Here we assess transcription of the BBA68 gene and production of BBA68 in infected mice and humans using real-time reverse transcriptase PCR and immunoblotting. The species specificity of FH binding to BBA68 was also tested. The data suggest that BBA68 does not play an important role in immune evasion in animals.

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页码：3030 / 3034

页数：5

相关论文

共 33 条

[1] Complement inhibitor factor H binding to Lyme disease spirochetes is mediated by inducible expression of multiple plasmid-encoded outer surface protein E paralogs [J].

Alitalo, A ;

Meri, T ;

Lankinen, H ;

Seppälä, L ;

Lahdenne, P ;

Hefty, PS ;

Akins, D ;

Meri, S .

JOURNAL OF IMMUNOLOGY, 2002, 169 (07) :3847-3853

[2] Complement regulator-acquiring surface protein 1 imparts resistance to human serum in Borrelia burgdorferi [J].

Brooks, CS ;

Vuppala, SR ;

Jett, AM ;

Alitalo, A ;

Meri, S ;

Akins, DR .

JOURNAL OF IMMUNOLOGY, 2005, 175 (05) :3299-3308

[3] Global analysis of Borrelia burgdorferi genes regulated by mammalian host-specific signals [J].

Brooks, CS ;

Hefty, PS ;

Jolliff, SE ;

Akins, DR .

INFECTION AND IMMUNITY, 2003, 71 (06) :3371-3383

[4] RpoS is not central to the general stress response in Borrelia burgdorferi but does control expression of one or more essential virulence determinants [J].

Caimano, MJ ;

Eggers, CH ;

Hazlett, KRO ;

Radolf, JD .

INFECTION AND IMMUNITY, 2004, 72 (11) :6433-6445

[5] Demonstration of OspC type diversity in invasive human Lyme disease isolates and identification of previously uncharacterized epitopes that define the specificity of the OspC murine antibody response [J].

Earnhart, CG ;

Buckles, EL ;

Dumler, JS ;

Marconi, RT .

INFECTION AND IMMUNITY, 2005, 73 (12) :7869-7877

[6] Analysis of promoter elements involved in the transcriptional initiation of RpoS-dependent Borrelia burgdorferi genes [J].

Eggers, CH ;

Caimano, MJ ;

Radolf, JD .

JOURNAL OF BACTERIOLOGY, 2004, 186 (21) :7390-7402

[7] Regulation of OspE-related, OspF-related, and Elp lipoproteins of Borrelia burgdorferi strain 297 by mammalian host-specific signals [J].

Hefty, PS ;

Jolliff, SE ;

Caimano, MJ ;

Wikel, SK ;

Radolf, JD ;

Akins, DR .

INFECTION AND IMMUNITY, 2001, 69 (06) :3618-3627

[8] The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi [J].

Hellwage, J ;

Meri, T ;

Heikkilä, T ;

Alitalo, A ;

Panelius, J ;

Lahdenne, P ;

Seppälä, IJT ;

Meri, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :8427-8435

[9] Selective binding of Borrelia burgdorferi OspE paralogs to factor H and serum proteins from diverse animals:: Possible expansion of the role of OspE in Lyme disease pathogenesis [J].

Hovis, KM ;

Tran, E ;

Sundy, CM ;

Buckles, E ;

McDowell, JV ;

Marconi, RT .

INFECTION AND IMMUNITY, 2006, 74 (03) :1967-1972

[10] Complement resistance of Borrelia burgdorferi correlates with the expression of BbCRASP-1, a novel linear plasmid-encoded surface protein that interacts with human factor H and FHL-1 and is unrelated to Erp proteins [J].

Kraiczy, P ;

Hellwage, J ;

Skerka, C ;

Becker, H ;

Kirschfink, M ;

Simon, MM ;

Brade, V ;

Zipfel, PF ;

Wallich, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (04) :2421-2429

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