Shell and small particles; Evaluation of new column technology

被引：61

作者：

Fekete, Szabolcs ^{[1
]}

Fekete, Jeno ^{[2
]}

Ganzler, Katalin ^{[1
]}

机构：

[1] Gedeon Richter Plc, Formulat Dev, Budapest, Hungary

[2] Budapest Univ Technol & Econ, Dept Inorgan & Analyt Chem, H-1111 Budapest, Hungary

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2009年 / 49卷 / 01期

关键词：

Column efficiency; Shell particles; Peak capacity; Kinetic plot; Ascentis Express; PERFORMANCE LIQUID-CHROMATOGRAPHY; PEAK-CAPACITY; SILICA PARTICLES; MASS-TRANSFER; POROSITY; KINETICS; POLYMER; HPLC;

D O I：

10.1016/j.jpba.2008.10.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 mu m particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities around 140-150 could be achieved in 25 min with the 5 cm long columns. The Ascentis Express column (packed with 2.7 mu m shell particles) showed similar efficiency to sub-2 mu m particles under gradient conditions. Applying isocratic separation, the column of 2.7 pm shell particles had a reduced plate height minimum of approximately h = 1.6. It was much smaller than obtained with totally porous particles (h approximate to 2.8). The impedance time also proved more favorable with 2.7 mu m shell particles than with totally porous particles. The influence of extra-column volume on column efficiency was investigated. The extra-column dispersion of the chromatographic system may cause a shift of the HETP curves. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：64 / 71

页数：8

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