Genome of an Endosymbiont Coupling N2 Fixation to Cellulolysis Within Protist Cells in Termite Gut

被引：161

作者：

Hongoh, Yuichi ^{[1
]}

Sharma, Vineet K. ^{[2
,3
]}

Prakash, Tulika ^{[2
,3
]}

Noda, Satoko ^{[1
]}

Toh, Hidehiro ^{[2
,3
]}

Taylor, Todd D. ^{[2
,3
]}

Kudo, Toshiaki ^{[1
]}

Sakaki, Yoshiyuki ^{[2
]}

Toyoda, Atsushi ^{[2
,4
]}

Hattori, Masahira ^{[2
,5
]}

Ohkuma, Moriya ^{[1
]}

机构：

[1] RIKEN, Adv Sci Inst, Ecomol Biorecycling Sci Res Team, Wako, Saitama 3510198, Japan

[2] RIKEN, Genom Sci Ctr, Kanagawa 2300045, Japan

[3] RIKEN, MetaSyst Res Team, Adv Sci Inst, Kanagawa 2300045, Japan

[4] Natl Inst Genet, Comparat Genom Lab, Mishima, Shizuoka 4118540, Japan

[5] Univ Tokyo, Dept Computat Biol, Grad Sch Frontier Sci, Chiba 2778561, Japan

来源：

SCIENCE | 2008年 / 322卷 / 5904期

基金：

日本学术振兴会;

关键词：

D O I：

10.1126/science.1165578

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Termites harbor diverse symbiotic gut microorganisms, the majority of which are as yet uncultivable and their interrelationships unclear. Here, we present the complete genome sequence of the uncultured Bacteroidales endosymbiont of the cellulolytic protist Pseudotrichonympha grassii, which accounts for 70% of the bacterial cells in the gut of the termite Coptotermes formosanus. Functional annotation of the chromosome ( 1,114,206 base pairs) unveiled its ability to fix dinitrogen and recycle putative host nitrogen wastes for biosynthesis of diverse amino acids and cofactors, and import glucose and xylose as energy and carbon sources. Thus, nitrogen fixation and cellulolysis are coupled within the protist's cells. This highly evolved symbiotic system probably underlies the ability of the worldwide pest termites Coptotermes to use wood as their sole food.

引用

页码：1108 / 1109

页数：2

共 18 条

[1] NITROGEN-FIXATION IN TERMITES [J].

BREZNAK, JA ;

BRILL, WJ ;

MERTINS, JW ;

COPPEL, HC .

NATURE, 1973, 244 (5418) :577-579

[2] Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis [J].

Carlton, Jane M. ;

Hirt, Robert P. ;

Silva, Joana C. ;

Delcher, Arthur L. ;

Schatz, Michael ;

Zhao, Qi ;

Wortman, Jennifer R. ;

Bidwell, Shelby L. ;

Alsmark, U. Cecilia M. ;

Besteiro, Sebastien ;

Sicheritz-Ponten, Thomas ;

Noel, Christophe J. ;

Dacks, Joel B. ;

Foster, Peter G. ;

Simillion, Cedric ;

Van de Peer, Yves ;

Miranda-Saavedra, Diego ;

Barton, Geoffrey J. ;

Westrop, Gareth D. ;

Mueller, Sylke ;

Dessi, Daniele ;

Fiori, Pier Luigi ;

Ren, Qinghu ;

Paulsen, Ian ;

Zhang, Hanbang ;

Bastida-Corcuera, Felix D. ;

Simoes-Barbosa, Augusto ;

Brown, Mark T. ;

Hayes, Richard D. ;

Mukherjee, Mandira ;

Okumura, Cheryl Y. ;

Schneider, Rachel ;

Smith, Alias J. ;

Vanacova, Stepanka ;

Villalvazo, Maria ;

Haas, Brian J. ;

Pertea, Mihaela ;

Feldblyum, Tamara V. ;

Utterback, Terry R. ;

Shu, Chung-Li ;

Osoegawa, Kazutoyo ;

de Jong, Pieter J. ;

Hrdy, Ivan ;

Horvathova, Lenka ;

Zubacova, Zuzana ;

Dolezal, Pavel ;

Malik, Shehre-Banoo ;

Logsdon, John M., Jr. ;

Henze, Katrin ;

Gupta, Arti .

SCIENCE, 2007, 315 (5809) :207-212

[3] Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell [J].

Hongoh, Yuichi ;

Sharma, Vineet K. ;

Prakash, Tulika ;

Noda, Satoko ;

Taylor, Todd D. ;

Kudo, Toshiaki ;

Sakaki, Yoshiyuki ;

Toyoda, Atsushi ;

Hattori, Masahira ;

Ohkuma, Moriya .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (14) :5555-5560

[4] Hydrogen production by termite gut protists:: Characterization of iron hydrogenases of parabasalian symbionts of the termite Coptotermes formosanus [J].

Inoue, Jun-Ichi ;

Saita, Kanako ;

Kudo, Toshiaki ;

Ui, Sadaharu ;

Ohkuma, Moriya .

EUKARYOTIC CELL, 2007, 6 (10) :1925-1932

[5] United States Department of Agriculture -: Agriculture Research Service -: research on targeted management of the Formosan subterranean termite Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) [J].

Lax, AR ;

La Osbrink, W .

PEST MANAGEMENT SCIENCE, 2003, 59 (6-7) :788-800

[6] Nitrogen fixation by symbiotic and free-living spirochetes [J].

Lilburn, TC ;

Kim, KS ;

Ostrom, NE ;

Byzek, KR ;

Leadbetter, JR ;

Breznak, JA .

SCIENCE, 2001, 292 (5526) :2495-2498

[7] Cospeciation in the triplex symbiosis of termite gut protists (Pseudotrichonympha spp.), their hosts, and their bacterial endosymbionts [J].

Noda, S. ;

Kitade, O. ;

Inoue, T. ;

Kawai, M. ;

Kanuka, M. ;

Hiroshima, K. ;

Hongoh, Y. ;

Constantino, R. ;

Uys, V. ;

Zhong, J. ;

Kudo, T. ;

Ohkuma, M. .

MOLECULAR ECOLOGY, 2007, 16 (06) :1257-1266

[8] Endosymbiotic Bacteroidales bacteria of the flagellated protist Pseudotrichonympha grassii in the gut of the termite Coptotermes formosanus [J].

Noda, S ;

Iida, T ;

Kitade, S ;

Nakajima, H ;

Kudo, T ;

Ohkuma, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8811-8817

[9]

Noda Satoko, 2002, Microbes and Environments, V17, P139, DOI 10.1264/jsme2.17.139

[10]

Ohkuma M, 1999, APPL ENVIRON MICROB, V65, P4926

← 1 2 →