The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves

被引:234
作者
Berger, Yael [1 ,2 ]
Harpaz-Saad, Smadar [1 ,2 ]
Brand, Arnon [1 ,2 ]
Melnik, Hadas [1 ,2 ]
Sirding, Neti [1 ,2 ]
Alvarez, John Paul [3 ]
Zinder, Michael [1 ,2 ]
Samach, Alon [1 ,2 ]
Eshed, Yuval [3 ]
Ori, Naomi [1 ,2 ]
机构
[1] Hebrew Univ Jerusalem, Fac Agr, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel
[2] Hebrew Univ Jerusalem, Fac Agr, Otto Warburg Minerva Ctr Agr Biotechnol, IL-76100 Rehovot, Israel
[3] Weizmann Inst Sci, Dept Plant Sci, IL-76100 Rehovot, Israel
来源
DEVELOPMENT | 2009年 / 136卷 / 05期
基金
以色列科学基金会;
关键词
CUC2; NAM; Boundary; Compound leaves; miR164; Tomato; RELATIVE CARDAMINE-HIRSUTA; SHOOT APICAL MERISTEM; SOLANUM-LYCOPERSICON; ARABIDOPSIS-THALIANA; MICRORNA REGULATION; ORGAN BOUNDARIES; AUXIN TRANSPORT; GENE-EXPRESSION; MUTANT; MORPHOGENESIS;
D O I
10.1242/dev.031625
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Leaves are formed at the flanks of the shoot apical meristem (SAM) and develop into a variety of forms. In tomato, prolonged leaf patterning enables the elaboration of compound leaves by reiterative initiation of leaflets with lobed margins. In goblet (gob) loss-of-function mutants, primary leaflets are often fused, secondary leaflets and marginal serrations are absent, and SAMs often terminate precociously. We show that GOB encodes a NAC-domain transcription factor expressed in narrow stripes at the leaf margins, flanking the distal side of future leaflet primordia, and at the boundaries between the SAM and leaf primordia. Leaf-specific overexpression of the microRNA miR164, a negative regulator of GOB-like genes, also leads to loss of secondary-leaflet initiation and to smooth leaflet margins. Plants carrying a dominant gob allele with an intact ORF but disrupted miR164 binding site produce more cotyledons and floral organs, have split SAMs and, surprisingly, simpler leaves. Overexpression of a form of GOB with an altered miR164 binding site in leaf primordia leads to delayed leaflet maturation, frequent, improperly timed and spaced initiation events, and a simple mature leaflet form owing to secondary-leaflet fusion. miR164 also affects leaflet separation in Cardamine hirsuta, a Brassicaceae species with complex leaves. Genetic and molecular analyses suggest that GOB expression is intact in the simplified leaves of entire tomato mutants, which have a defect in a putative repressor of auxin responses. Our results show that GOB marks leaflet boundaries and that its accurate spatial, temporal and quantitative activity affects leaf elaboration in a context-dependent manner.
引用
收藏
页码:823 / 832
页数:10
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