Cloning and characterisation of the chicken gene encoding the telomeric protein TRF2

被引：18

作者：

Konrad, JP

Mills, W

Easty, DJ

Farr, CJ

机构：

[1] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England

[2] Univ Coll Dublin, Ctr Biotechnol, Lab 1 G08, Dublin 4, Ireland

来源：

GENE | 1999年 / 239卷 / 01期

基金：

英国惠康基金;

关键词：

dimerisation domain; myb-related DNA binding motif; telobox; telomere; (TTAGGG)n; vertebrate;

D O I：

10.1016/S0378-1119(99)00374-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The telomere-associated protein TRF2 binds as a homodimer to double-stranded (TTAGGG)n arrays in vitro and localises to chromosome ends in vivo. Inhibition of TRF2 in human cell lines and recent electron microscopy analyses suggest that TRF2 plays a crucial role in the maintenance of telomere integrity. To study the role of TRF2 in vertebrate telomere biology using an alternative model system, we report the isolation and characterisation of the chicken TRF2 locus. The TRF2 protein is highly conserved between mammals and birds, particularly within the dimerisation and myb-type DMA binding domains. However, the chicken ORF predicts an additional protein domain consisting of 15 copies of a degenerate 13 amino acid repeat. Indirect immunofluorescence reveals the localisation of a FLAG-tagged version of the chicken TRF2 protein at chromosome ends in both chicken and human cells suggesting that the protein is functionally conserved. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：81 / 90

页数：10

共 27 条

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