The post-pollination ethylene burst and the continuation of floral advertisement are harbingers of non-random mate selection in Nicotiana attenuata

被引:27
作者
Bhattacharya, Samik [1 ]
Baldwin, Ian T. [1 ]
机构
[1] Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany
关键词
Nicotiana attenuata; non-random mate selection; pollen tube competition; post-pollination ethylene burst; floral advertisement; seed paternity; female choice; male-male competition; POLLEN-TUBE GROWTH; POLLINATION-INDUCED SENESCENCE; RECEPTOR KINASE LEPRK2; PISTIL INTERACTIONS; PETUNIA-HYBRIDA; 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE; TRANSMITTING TISSUE; SEED PATERNITY; FLOWERS; PLANTS;
D O I
10.1111/j.1365-313X.2012.05011.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The self-compatible plant Nicotiana attenuata grows in genetically diverse populations after fires, and produces flowers that remain open for 3 days and are visited by assorted pollinators. To determine whether and when post-pollination non-random mate selection occurs among self and non-self pollen, seed paternity and semi-in vivo pollen tube growth were determined in controlled single/mixed pollinations. Despite all pollen sources being equally proficient in siring seeds in single-genotype pollinations, self pollen was consistently selected in mixed pollinations, irrespective of maternal genotype. However, clear patterns of mate discrimination occurred amongst non-self pollen when mixed pollinations were performed soon after corollas open, including selection against hygromycin B resistance (transformation selectable marker) in wild-type styles and for it in transformed styles. However, mate choice among pollen genotypes was completely shut down in plants transformed to be unable to produce (irACO) or perceive (ETR1) ethylene. The post-pollination ethylene burst, which originates primarily from the stigma and upper style, was strongly correlated with mate selection in single and mixed hand-pollinations using eight pollen donors in two maternal ecotypes. The post-pollination ethylene burst was also negatively correlated with the continuation of emission of benzylacetone, the most abundant pollinator-attracting corolla-derived floral volatile. We conclude that ethylene signaling plays a pivotal role in mate choice, and the post-pollination ethylene burst and the termination of benzylacetone release are accurate predictors, both qualitatively and quantitatively, of pre-zygotic mate selection and seed paternity.
引用
收藏
页码:587 / 601
页数:15
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