Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area

被引：44

作者：

Björke, H ^{[1
]}

Andersson, K ^{[1
]}

机构：

[1] Uppsala Univ, Div Biomed Radiat Sci, Uppsala, Sweden

来源：

APPLIED RADIATION AND ISOTOPES | 2006年 / 64卷 / 01期

关键词：

radioligand; affinity; cell-based; interaction;

D O I：

10.1016/j.apradiso.2005.06.007

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity-reference activity). The affinities obtained for different ligand-receptor interactions agreed with affinities reported in the literature. (c) 2005 Elsevier Ltd. All rights reserved.

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收藏

页码：32 / 37

页数：6

相关论文

共 11 条

[1]

A novel cell-based scintillation proximity assay for studying protein function and activity in vitro using membrane-soluble scintillants [J].

Culliford, SJ ;

McCauley, P ;

Sutherland, AJ ;

McCairn, M ;

Sutherland, J ;

Blackburn, J ;

Kozlowski, RZ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (04) :857-863

[2]

SURFACE-PLASMON RESONANCE DETECTION AND MULTISPOT SENSING FOR DIRECT MONITORING OF INTERACTIONS INVOLVING LOW-MOLECULAR-WEIGHT ANALYTES AND FOR DETERMINATION OF LOW AFFINITIES [J].

KARLSSON, R ;

STAHLBERG, R .

ANALYTICAL BIOCHEMISTRY, 1995, 228 (02) :274-280

[3]

A structure-based model for ligand binding and dimerization of EGF receptors [J].

Klein, P ;

Mattoon, D ;

Lemmon, MA ;

Schlessinger, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (04) :929-934

[4]

The 6H1 orphan receptor, claimed to be the p2y5 receptor, does not mediate nucleotide-promoted second messenger responses [J].

Li, Q ;

Schachter, JB ;

Harden, TK ;

Nicholas, RA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 236 (02) :455-460

[5]

LI Q, 1997, BIOCHEM BIOPH RES CO, V18, P236

[6]

Minor LK, 2003, CURR OPIN DRUG DISC, V6, P760

[7]

DOTA-lanreotide: A novel somatostatin analog for tumor diagnosis and therapy [J].

Smith-Jones, PM ;

Bischof, C ;

Leimer, M ;

Gludovacz, D ;

Angelberger, P ;

Pangerl, T ;

Peck-Radosavljevic, M ;

Hamilton, G ;

Kaserer, K ;

Kofler, A ;

Schlagbauer-Wadl, H ;

Traub, T ;

Virgolini, I .

ENDOCRINOLOGY, 1999, 140 (11) :5136-5148

[8]

[111In]Bz-DTPA-hEGF:: Preparation and in vitro characterization of a potential anti-glioblastoma targeting agent [J].

Sundberg, AL ;

Orlova, A ;

Bruskin, A ;

Gedda, L ;

Carlsson, J ;

Blomquist, E ;

Lundqvist, H ;

Tolmachev, V .

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2003, 18 (04) :643-654

[9]

Combined effect of gefitinib ('Iressa', ZD1839) and targeted radiotherapy with 211At-EGF [J].

Sundberg, ÅL ;

Almqvist, Y ;

Orlova, A ;

Blomquist, E ;

Jensen, HJ ;

Gedda, L ;

Tolmachev, V ;

Carlsson, J .

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2003, 30 (10) :1348-1356

[10]

Selection and characterization of HER2/neu-binding affibody ligands [J].

Wikman, M ;

Steffen, AC ;

Gunneriusson, E ;

Tolmachev, V ;

Adams, GP ;

Carlsson, J ;

Ståhl, S .

PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (05) :455-462