An insight into iTRAQ: where do we stand now?

被引：336

作者：

Evans, Caroline ^{[3
]}

Noirel, Josselin ^{[3
]}

Ow, Saw Yen ^{[3
]}

Salim, Malinda ^{[3
]}

Pereira-Medrano, Ana G. ^{[3
]}

Couto, Narciso ^{[3
]}

Pandhal, Jagroop ^{[3
]}

Smith, Duncan ^{[2
]}

Trong Khoa Pham ^{[3
]}

Karunakaran, Esther ^{[3
]}

Zou, Xin ^{[3
]}

Biggs, Catherine A. ^{[3
]}

Wright, Phillip C. ^{[1
,3
]}

机构：

[1] Univ Sheffield, ChELSI Inst, Dept Chem & Biol Engn, Mappin St, Sheffield S1 3JD, S Yorkshire, England

[2] Univ Manchester, Manchester Paterson Inst Canc Res, MBCF Mass Spectrometry Facil, Manchester M20 4BX, Lancs, England

[3] Univ Sheffield, ChELSI Inst, Biol & Environm Syst Grp, Sheffield S1 3JD, S Yorkshire, England

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2012年 / 404卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

Shotgun proteomics; iTRAQ; Ratio underestimation; Relative quantification; MS/MS instrumentation; Isotopic impurities; Statistical validation; Modelling; PTMs; Label-free; QUANTITATIVE PROTEOMIC ANALYSIS; MULTIPLEXED PROTEIN QUANTITATION; SITE-SPECIFIC QUANTIFICATION; DATA-INDEPENDENT ANALYSIS; RESIN-ASSISTED CAPTURE; MASS-SPECTROMETRY DATA; ISOBARIC TAGS; LABELED PEPTIDES; LTQ-ORBITRAP; RELATIVE QUANTIFICATION;

D O I：

10.1007/s00216-012-5918-6

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The iTRAQ (isobaric tags for relative and absolute quantification) technique is widely employed in proteomic workflows requiring relative quantification. Here, we review the iTRAQ literature; in particular, we focus on iTRAQ usage in relation to other commonly used quantitative techniques e.g. stable isotope labelling in culture (SILAC), label-free methods and selected reaction monitoring (SRM). As a result, we identify several issues arising with respect to iTRAQ. Perhaps frustratingly, iTRAQ's attractiveness has been undermined by a number of technical and analytical limitations: it may not be truly quantitative, as the changes in abundance reported will generally be underestimated. We discuss weaknesses and strengths of iTRAQ as a methodology for relative quantification in the light of this and other technical issues. We focus on technical developments targeted at iTRAQ accuracy and precision, use of 4-plex over 8-plex reagents and application of iTRAQ to post-translational modification (PTM) workflows. We also discuss iTRAQ in relation to label-free approaches, to which iTRAQ is losing ground.

引用

页码：1011 / 1027

页数：17

共 113 条

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[2]

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