Evolving trends in the dereplication of natural product extracts: New methodology for rapid, small-scale investigation of natural product extracts

被引：126

作者：

Lang, Gerhard ^{[1
]}

Mayhudin, Nor Ainy ^{[1
,2
]}

Mitova, Maya I. ^{[1
]}

Sun, Lin ^{[1
]}

van der Sar, Sonia ^{[1
]}

Blunt, John W. ^{[1
]}

Cole, Anthony L. J. ^{[2
]}

Ellis, Gill ^{[1
]}

Laatsch, Hartmut ^{[3
]}

Munro, Murray H. G. ^{[1
]}

机构：

[1] Univ Canterbury, Dept Chem, Christchurch 1, New Zealand

[2] Univ Canterbury, Sch Biol Sci, Christchurch 1, New Zealand

[3] Univ Gottingen, Dept Organ & Biomol Chem, Gottingen, Germany

来源：

JOURNAL OF NATURAL PRODUCTS | 2008年 / 71卷 / 09期

关键词：

D O I：

10.1021/np8002222

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of H-1 NMR spectroscopy as a definitive dereplication tool.

引用

页码：1595 / 1599

页数：5

共 29 条

[1]

[Anonymous], DICT NATURAL PRODUCT

[2]

BERDY J, 2000, CRC HDB BIOACTIVE MI

[3] Accelerated dereplication of natural products, supported by reference libraries [J].

Bitzer, Jens ;

Koepcke, Baerbel ;

Stadler, Marc ;

Heilwig, Veronika ;

Ju, Yu-Ming ;

Seip, Stephan ;

Henkel, Thomas .

CHIMIA, 2007, 61 (06) :332-338

[4]

Blunt J., 2007, ANTIMARIN DATABASE

[5]

BLUNT JW, 2000, INT CHEM C PAC BAS S

[6]

BLUNT JW, 2007, 5 EUR C MAR NAT PROD

[7]

BLUNT JW, 2007, AS MYC C 2007 10 INT

[8]

BLUNT JW, 2007, 2 CMDD S MAR NAT PRO

[9]

BLUNT JW, 2006, 47 ANN M AM SOC PHAR

[10]

BLUNT JW, 2005, PAC 2005 INT CHEM C

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