Evolving trends in the dereplication of natural product extracts: New methodology for rapid, small-scale investigation of natural product extracts

被引：126

作者：

Lang, Gerhard ^{[1
]}

Mayhudin, Nor Ainy ^{[1
,2
]}

Mitova, Maya I. ^{[1
]}

Sun, Lin ^{[1
]}

van der Sar, Sonia ^{[1
]}

Blunt, John W. ^{[1
]}

Cole, Anthony L. J. ^{[2
]}

Ellis, Gill ^{[1
]}

Laatsch, Hartmut ^{[3
]}

Munro, Murray H. G. ^{[1
]}

机构：

[1] Univ Canterbury, Dept Chem, Christchurch 1, New Zealand

[2] Univ Canterbury, Sch Biol Sci, Christchurch 1, New Zealand

[3] Univ Gottingen, Dept Organ & Biomol Chem, Gottingen, Germany

来源：

JOURNAL OF NATURAL PRODUCTS | 2008年 / 71卷 / 09期

关键词：

D O I：

10.1021/np8002222

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of H-1 NMR spectroscopy as a definitive dereplication tool.

引用

页码：1595 / 1599

页数：5

共 29 条

[11]

BLUNT JW, 2005, 4 EUR C MAR NAT PROD

[12] A rapid and facile method for the dereplication of purified natural products [J].

Bradshaw, J ;

Butina, D ;

Dunn, AJ ;

Green, RH ;

Hajek, M ;

Jones, MM ;

Lindon, JC ;

Sidebottom, PJ .

JOURNAL OF NATURAL PRODUCTS, 2001, 64 (12) :1541-1544

[13] An MS/MS library on an ion-trap instrument for efficient dereplication of natural products.: Different fragmentation patterns for [M+H]+ and [M+Na]+ ions [J].

Fredenhagen, A ;

Derrien, C ;

Gassmann, E .

JOURNAL OF NATURAL PRODUCTS, 2005, 68 (03) :385-391

[14] Application of capillary-scale NMR for the structure determination of phytochemicals [J].

Hu, JF ;

Garo, E ;

Yoo, HD ;

Cremin, PA ;

Zeng, L ;

Goering, MG ;

O'Neil-Johnson, M ;

Eldridge, GR .

PHYTOCHEMICAL ANALYSIS, 2005, 16 (02) :127-133

[15]

Koehn Frank E., 2008, V65

[16] Molecular formula analysis by an MS/MS/MS technique to expedite dereplication of natural products [J].

Konishi, Yasuo ;

Kiyota, Taira ;

Draghici, Cristina ;

Gao, Jin-Ming ;

Yeboah, Faustinus ;

Acoca, Stephane ;

Jarussophon, Suwatchai ;

Purisima, Enrico .

ANALYTICAL CHEMISTRY, 2007, 79 (03) :1187-1197

[17] BIOLOGICALLY-ACTIVE METABOLITES FROM FUNGI .6. PHOMOSINES-A-C 3 NEW BIARYL ETHERS FROM PHOMOPSIS SP [J].

KROHN, K ;

MICHEL, A ;

ROMER, E ;

FLORKE, U ;

AUST, HJ ;

DRAEGER, S ;

SCHULZ, B ;

WRAY, V .

NATURAL PRODUCT LETTERS, 1995, 6 (04) :309-314

[18]

LAATSCH H, 2007, ANTIBASE DATA BASE R

[19] Identification of natural products using HPLC-SPE combined with CapNMR [J].

Lambert, Maja ;

Wolfender, Jean-Luc ;

Staerk, Dan ;

Christensen, Brogger ;

Hostettmann, Kurt ;

Jaroszewski, Jerzy W. .

ANALYTICAL CHEMISTRY, 2007, 79 (02) :727-735

[20] Bioactivity profiling using HPLC/microtiter-plate analysis:: Application to a New Zealand marine alga-derived fungus, Gliocladium sp. [J].

Lang, G ;

Mitova, MI ;

Ellis, G ;

Van der Sar, S ;

Phipps, RK ;

Blunt, JW ;

Cummings, NJ ;

Cole, ALJ ;

Munro, MHG .

JOURNAL OF NATURAL PRODUCTS, 2006, 69 (04) :621-624

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