Single-molecule detection fluorescence of surface-bound species in vacuum

被引：83

作者：

Weston, KD ^{[1
]}

Carson, PJ ^{[1
]}

DeAro, JA ^{[1
]}

Buratto, SK ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 308卷 / 1-2期

关键词：

D O I：

10.1016/S0009-2614(99)00553-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have performed single-molecule fluorescence detection of DiIC(12) molecules adsorbed on a solid substrate where the surrounding pressure is controlled. The triplet lifetimes are significantly longer (4-100 ms) and far more easily observed at moderate vacuum pressures than in air(typically < 100 mu s) The photobleaching rate also decreases by over an order of magnitude in vacuum. In addition, we have observed fluctuations in the intersystem crossing rate and the triplet lifetime for a single molecule, consistent with our previous observations, in this same system in air, of hopping between two or more molecular configurations, each with its own time-averaged intensity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：58 / 64

页数：7

共 20 条

[1] DIRECT SPECTROSCOPIC OBSERVATION OF QUANTUM JUMPS OF A SINGLE-MOLECULE [J].

BASCHE, T ;

KUMMER, S ;

BRAUCHLE, C .

NATURE, 1995, 373 (6510) :132-134

[2] PHOTON ANTIBUNCHING IN THE FLUORESCENCE OF A SINGLE DYE MOLECULE TRAPPED IN A SOLID [J].

BASCHE, T ;

MOERNER, WE ;

ORRIT, M ;

TALON, H .

PHYSICAL REVIEW LETTERS, 1992, 69 (10) :1516-1519

[3] PHOTON BUNCHING IN THE FLUORESCENCE FROM SINGLE MOLECULES - A PROBE FOR INTERSYSTEM CROSSING [J].

BERNARD, J ;

FLEURY, L ;

TALON, H ;

ORRIT, M .

JOURNAL OF CHEMICAL PHYSICS, 1993, 98 (02) :850-859

[4]

CARSON PJ, IN PRESS

[5] On/off blinking and switching behaviour of single molecules of green fluorescent protein [J].

Dickson, RM ;

Cubitt, AB ;

Tsien, RY ;

Moerner, WE .

NATURE, 1997, 388 (6640) :355-358

[6] Quantum jumps of single molecules at room temperature [J].

Ha, T ;

Enderle, T ;

Chemla, DS ;

Selvin, PR ;

Weiss, S .

CHEMICAL PHYSICS LETTERS, 1997, 271 (1-3) :1-5

[7] Hindered rotational diffusion and rotational jumps of single molecules [J].

Ha, T ;

Glass, J ;

Enderle, T ;

Chemla, DS ;

Weiss, S .

PHYSICAL REVIEW LETTERS, 1998, 80 (10) :2093-2096

[8] Single molecule dynamics studied by polarization modulation [J].

Ha, T ;

Enderle, T ;

Chemla, DS ;

Selvin, PR ;

Weiss, S .

PHYSICAL REVIEW LETTERS, 1996, 77 (19) :3979-3982

[9]

Loudon R., 1983, QUANTUM THEORY LIGHT

[10] Single-molecule spectral fluctuations at room temperature [J].

Lu, HP ;

Xie, XS .

NATURE, 1997, 385 (6612) :143-146

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