Specialization and targeting of B-type cyclins

被引：90

作者：

Cross, FR ^{[1
]}

Yuste-Rojas, M ^{[1
]}

Gray, S ^{[1
]}

Jacobson, MD ^{[1
]}

机构：

[1] Rockefeller Univ, New York, NY 10021 USA

来源：

MOLECULAR CELL | 1999年 / 4卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(00)80183-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The B-type cyclins of S. cerevisiae are diversified with respect to time of expression during the cell cycle as well as biological function. We replaced the early-expressed CLB5 coding sequence with the late-expressed CLB2 coding sequence, at the CLB5 locus. CLB5::CLB2 exhibited almost no rescue of clb5-specific replication defects, although it could rescue clb1 clb2 lethality, and in synchronized cells Clb2p-associated kinase activity from CLB5::CLB2 rose early in the cell cycle, similar to that of Clb5p. Mutagenesis of a potential substrate-targeting domain of CLB5 reduced biological activity without reducing Clb5p-associated kinase activity. Thus, Clb5p may have targeting domains required for CLB5-specific biological activity.

引用

页码：11 / 19

页数：9