BUL: A novel lectin from Bauhinia ungulata L. seeds with fungistatic and antiproliferative activities

被引：36

作者：

Silva, Helton C. ^{[1
]}

Pinto, Luciano da S. ^{[2
]}

Teixeira, Edson H. ^{[3
]}

Nascimento, Kyria S. ^{[1
]}

Cavada, Benildo S. ^{[1
]}

Silva, Andre Luis C. ^{[4
]}

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Mol Biol Ativas Biomol Lab, BR-60455970 Fortaleza, CE, Brazil

[2] Univ Fed Pelotas, Ctr Biotecnol, BR-96010900 Pelotas, RS, Brazil

[3] Univ Fed Ceara, Dept Pathol & Legal Med, Fac Med, Integrated Lab Biomol LIBS, BR-60430160 Fortaleza, CE, Brazil

[4] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Biotecnol Mol LabBMol, BR-60455970 Fortaleza, CE, Brazil

来源：

PROCESS BIOCHEMISTRY | 2014年 / 49卷 / 02期

关键词：

Lectin; Bauhinia ungulata; Fungistatic and antiproliferative activities; GALACTOSE-SPECIFIC LECTIN; PLANT-LECTINS; MILLIGRAM QUANTITIES; GROWTH-INHIBITION; ANTICANCER DRUG; PURIFICATION; PURPUREA; AGGLUTININ; PROTEINS; BINDING;

D O I：

10.1016/j.procbio.2013.10.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A new galactose-binding lectin, termed BUL, has been purified from seeds of Bauhinia ungulata L (Caesalpinoideae) by precipitation with solid ammonium sulfate followed by agarose-lactose affinity chromatography. B. ungulata lectin strongly agglutinated rabbit erythrocytes, both native and treated with proteolytic enzymes, and was inhibited by D-galactose and D-galactose-derived sugars, especially N-acetyl-D-galactosamine. BUL was shown to be a stable glycoprotein, maintaining its hemagglutinating activity after incubation at wide ranges of temperature and pH, but not after incubation with EDTA. By SOS-PAGE analysis, purified BUL showed an electrophoretic profile consisting of a single band with apparent molecular mass of 30 kDa. BUL showed intrinsic fluorescence typical of folded globular proteins, and CD spectra of lectin in the native state showed a predominance of I3-sheet secondary structure. The N-terminal amino acid sequence of 19 residues showed a high sequential similarity to other galactose-specific lectins from the Bauhinia genus. In addition, BUL showed antifungal activity against phytopathogenic species and showed in vitro antiproliferative activity against the HT-29 cell line of human colon adenocarcinoma in a dose-dependent manner. (c) 2013 Elsevier Ltd. All rights reserved.

引用

页码：203 / 209

页数：7

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