Probing the affinity and specificity of yeast alcohol dehydrogenase I for coenzymes

被引:23
作者
Fan, F [1 ]
Plapp, BV [1 ]
机构
[1] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA
基金
美国国家科学基金会;
关键词
nucleotide binding; NAD; NADP; mutagenesis; enzyme kinetics;
D O I
10.1006/abbi.1999.1242
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD(+) and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding pocket of SceADH were substituted in attempts to improve affinity for coenzymes or reactivity with NADP, Substitutions in SceADH (Gly202Ile or Ser246Ile) with the corresponding residues in the adenine binding site of the homologous EcaADH have modest effects on coenzyme binding and other kinetic constants, but the Ser246Ile substitution decreases turnover numbers by 350-fold. The Ser176Phe substitution (also near adenine site) significantly decreases affinity for coenzymes and turnover numbers, In the consensus nucleotide-binding beta alpha beta fold sequence, SceADH has two alanine residues (177-G<(AA)under bar>GGLG-183) instead of the Leu200 in EcaADH (199-G (L) under bar GGVG-204); the Ala178-Ala179 to Leu substitution significantly decreases affinity for coenzymes and turnover numbers. Some NADP-dependent enzymes have an Ala corresponding to Gly183 in SceADH; the Gly183Ala substitution significantly decreases affinity for coenzymes and turnover numbers. NADP-dependent enzymes usually have a neutral residue instead of the Asp (Asp201 in SceADH) that interacts with the hydroxyl groups of the adenosine ribose, along with a basic residue (at position 202 or 203) to stabilize the S'-phosphate of NADP, The Gly203Arg change in SceADH does not significantly affect the kinetics. The Gly183Ala or Gly203Arg substitutions do not enable SceADH to use NADP(+) as coenzyme, SceADH with the single Asp201Gly or double Asp201Gly:Gly203Arg substitutions have similar, low activity with NADP(+), The results suggest that several of the amino acid residues participate in coenzyme binding and that conversion of specificity for coenzyme requires multiple substitutions. (C) 1999 Academic Press.
引用
收藏
页码:240 / 249
页数:10
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