GFP-labelled Rubisco and aspartate aminotransferase are present in plastid stromules and traffic between plastids

被引：61

作者：

Kwok, EY ^{[1
]}

Hanson, MR ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2004年 / 55卷 / 397期

关键词：

aspartate aminotransferase; chloroplast; FRAP; photobleaching; plastid; protein trafficking; Rubisco; stromule;

D O I：

10.1093/jxb/erh062

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plastid stromules are membrane-bound protrusions of the plastid envelope that contain soluble stroma. Stromules are often found connecting plastids within a cell and fluorescence recovery after photobleaching (FRAP) experiments have demonstrated that green fluorescent protein (GFP) can move between plastids via these connections. In this report, the ability of endogenous plastid proteins to travel through stromules was investigated. The motility of GFP-labelled plastid aspartate aminotransferase and the Rubisco small subunit was studied in stromules by FRAP. Both fusion proteins assemble into protein complexes that appear to behave similarly to their endogenous counterparts. In addition, both enzymes are capable of trafficking between plastids via stromules.

引用

页码：595 / 604

页数：10

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